ITS Sequencing of Indian Isolates of <I>Lecanicillium</I> Species

B. Ramanujam; M. Balachander; G. Roopa; R. Rangeshwaran; Pritam Karmakar

doi:10.18311/jbc/2011/3726

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Authors

B. Ramanujam
National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Bangalore 560 024, Karnataka ,IN
M. Balachander
National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Bangalore 560 024, Karnataka ,IN
G. Roopa
National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Bangalore 560 024, Karnataka ,IN
R. Rangeshwaran
National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Bangalore 560 024, Karnataka ,IN
Pritam Karmakar
National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Bangalore 560 024, Karnataka ,IN

DOI:

https://doi.org/10.18311/jbc/2011/3726

Keywords:

ITS Region, Phylogenetic Analysis, Lecanicillium sp.

Abstract

The genetic diversity of thirty one isolates of Lecanicillium species isolated from insect hosts from various geographical regions of India were studied. Their phylogenetic relationships were determined using internal transcribed spacer, ITS1, ITS2 and 5.8S gene of rRNA sequence. Based on the sequence similarity of ITS region and construction of subsequent phylogenetic analysis (neighbour-joining method), 15 isolates were grouped as Lecanicillium lecanii, 11 isolates as L. attenuatum, 3 isolates as L. longisporum and 2 isolates as L. muscarium.