In vitro studies on the development of microbial consortia for the management of major diseases in coconut and citrus

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Authors

  • V. GOVARDHAN RAO
     AICRP on Palms, Horticultural Research Station, Dr. Y.S.R. Horticultural University, Ambajipeta – 533214, Andhra Pradesh ,IN
  • B. NEERAJA
     AICRP on Palms, Horticultural Research Station, Dr. Y.S.R. Horticultural University, Ambajipeta – 533214, Andhra Pradesh ,IN
  • N. B. V. CHALAPATHIRAO
     AICRP on Palms, Horticultural Research Station, Dr. Y.S.R. Horticultural University, Ambajipeta – 533214, Andhra Pradesh ,IN
  • T. RAJASHEKARAM
     Citrus Research Station, Dr. Y.S.R. Horticultural University, Tirupathi – 517502, Andhra Pradesh ,IN
  • A. KIREETI
     AICRP on Palms, Horticultural Research Station, Dr. Y.S.R. Horticultural University, Ambajipeta – 533214, Andhra Pradesh ,IN
  • V. ANOOSHA
     AICRP on Palms, Horticultural Research Station, Dr. Y.S.R. Horticultural University, Ambajipeta – 533214, Andhra Pradesh ,IN

DOI:

https://doi.org/10.18311/jbc/2024/43762

Keywords:

Basal stem rot, bud rot, coconut and citrus diseases, microbial consortia, leaf blight

Abstract

Microbial consortia for disease suppression involve combining multiple beneficial microorganisms to enhance their effectiveness in plant disease management. In present study, development of microbial consortia for the management of major diseases in coconut and citrus was carried out using bacteria Pseudomonas fluorescens, Pseudomonas putida (striata), Bacillus subtilis and fungi – Trichoderma reesei, T. harzianum, T. asperellum against major pathogens viz. Ganoderma lucidum, Thielaviopsis paradoxa, Phytopthora palmivora, Lasiodiplodia theobromae isolated from the coconut rhizosphere, and Fusarium solani isolated from the citrus rhizosphere. The promising fungal and bacterial antagonists were identified and studied for compatibility. Non-volatile compounds of consortia inhibited the test pathogens with an increase in concentration from 10 % to 75% with fungal consortia and bacterial consortia and also with mixed consortia which is composed of bacterial consortia + fungal consortia. Superior growth suppression was recorded with mixed consortia even at 10% concentration (59.44% to 65.83%) against the test pathogens in the ascending order of L. theobromae (59.44%) T. paradoxa (63.89%), G. lucidum (65.83%), P. palmivora (63.61%) and F. solani (62.78%). A similar trend was observed in 75% concentration where inhibition observed in the order of Thielaviopsis paradoxa (90.28%), G. lucidum (89.44%), F. solani (82.50%), L. theobromae (81.94%) and P. palmivora (81.39%). Volatile effect by bacterial consortia recorded the superior inhibition on test pathogens in the order of Ganoderma (85.28%), F. solani (75.28%), T. paradoxa (71.94%), P. palmivora (71.67%) and L. theobrome (67.50%) compared to the individual bioagents. Similarly, the fungal consortia showed the superior inhibitory effect on test pathogens in the order of G. lucidum (83.25%), P. palmivora (82.50%), L. theobromae (83.06%), F. solani (80.56%) and T. paradoxa (73.61%). Since there was no zone of inhibition between the strains, the interactions between Pseudomonas and Bacillus strains of Trichoderma spp. were compatible with one another. Neem cake recorded superior CFU population from 9.43 X 106 CFU at seven days by T. asperellum. Shelf life study on mixed consortia with bacterial + fungal bioagents in talc formulation indicated that all the bacterial and fungal CFU count recorded in 106 dilution for 90 days.

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Published

2024-10-08

How to Cite

RAO, V. G., NEERAJA, B., CHALAPATHIRAO, N. B. V., RAJASHEKARAM, T., KIREETI, A., & ANOOSHA, V. (2024). <i>In vitro</i> studies on the development of microbial consortia for the management of major diseases in coconut and citrus. Journal of Biological Control, 38(3), 284–301. https://doi.org/10.18311/jbc/2024/43762

Issue

Volume 38, Issue 3, September 2024

Section

Research Articles

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Copyright (c) 2024 V. GOVARDHAN RAO, B. NEERAJA, N. B. V. CHALAPATHIRAO, T. RAJASHEKARAM, A. KIREETI, V. ANOOSHA (Author)

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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Received 2024-04-24
Accepted 2024-09-16
Published 2024-10-08

 

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