Standardization of Physiological Requirements, Substrates and Carrier Materials for Mass Production of Bacillus subtilis
DOI:
https://doi.org/10.18311/jbc/2015/6079Keywords:
Bacillus subtilis, Carrier Material, Media, Temperature.Abstract
Optimization of Bacillus subtilis growth conditions for mass production under laboratory conditions was investigated as part of a biological control programme. Aspects such as increasing yield using various culture media, pH, temperatures and carrier materials were studied. Nutrient broth (100í—108) and 20 g molasses and 10 g yeast extract based media (98.60í—108) gave the highest yield and 20 g molasses and 10g yeast extract broth was the most economical. pH of 7-8, temperature of 30-350C and talc as carrier material were proved to best for mass production of B. subtilis.
Downloads
Metrics
Downloads
Published
How to Cite
Issue
Section
References
Harwood CR. 1989, Introduction to the biotechnology of Bacillus. In: C.R. Harwood (Ed.). Biotechnology Handbooks, vol 2: Bacillus. 1 - 4. Plenum Press, London.
Korsten L., Cook N. 1996. Optimizing culturing conditions for Bacillus subtilis. South African Avacado Growers Association Yearbook 19: 54-58.
Nakkeeran S, Kavitha K, Chandrasekar G, Renukadevi P, Fernando WGD. 2006. Induction plant defense compounds by Pseudomonas chlororaphis PA23 and Bacillus subtilis BSCBE4 in controlling damping off of hot pepper caused by Pythium aphanidermatum. Biocontrol Sci Technol. 16 (4): 403- 416.
Norris JR, Berkeley RCW, Logan NA, Donnel AG. 1981. The genera Bacillus and Sporolactobacillus. p 71111742. In: M. Starr, H. Stolp, H.G. Truper, A. Balows and H. Starr M, Stolp H, Truper HG, Balows A, Schlegel H. (Eds) The Prokaryotes: A handbook on habitats, isolation, and identification of bacteria, vol 2: 1711 - 1742. Springer-Verlag, Berlin.
Peighmi-Ashnari S, Shariti-Tehrani A, Ahmadzadeh M, Behhoudi K. 2009. Interaction of different media on production and bioefficacy of Psuedomonas fluorescens P-35 and Bacillus subtilis B-3 against grey mold of apple. J Pl Pathol. 91 (1): 65-70.
Sharp J, Scawen MD, Atkinson T. 1989. Fermentation and downstream processing oí Bacillus. p 255-291 In: Harwood CR. (Ed.). Biotechnology handbooks, vol 2 Bacillus. Plenum Press, London.
Soleiman G, Masoud A. 2013. Optimization of a cost effective culture medium for the large scale production of Bacillus subtilis UTB96. Archives Phytopath Pl Prot 46: 1552-1563.