Immunohistochemical Localization of Androgen, Meletonin and Glucocorticoid Receptors in the Testis of Indian Pygmy Field Mouse Mus terricolor

Jump To References Section

Authors

  • Sweta Arora
     Mahila Mahavidyalaya, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh ,IN
  • Poonam Singh
     Mahila Mahavidyalaya, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh ,IN
  • Priyoneel Basu
     Behavioural Neuroscience Unit, Department of Psychology, Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta ,CA
  • Chandana Haldar
     Pineal Research Laboratory, Department of Zoology – Centre of Advanced Study, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh ,IN

DOI:

https://doi.org/10.18311/jer/2018/24389

Keywords:

Immunohistochemistry, Mus terricolor, Receptors, Seasonal Reproduction
Reproductive Biology

Abstract

Testosterone, melatonin and glucocorticoids are known to mediate their actions through their respective receptors, i.e., Androgen Receptor (AR), Melatonin Receptor (MT1) and Glucocorticoid Receptor (GR), endowed on the target tissues. The gonads of M. terricolor responded positively to administration of these hormones, suggesting the possibility of a direct action of these hormones on the gonads through their respective receptors. The present study was, therefore, undertaken to localize the Androgen (AR), Melatonin (MT1) and Glucocorticoid (GR) receptors in the testis of this rodent adopting immunohistochemistry as the tool. The result indicated that AR is expressed in the Leydig cells and germ cells of the testis of M. terricolor. MT1 receptor is localized in the Leydig cells, peritubular myoid cells, and endothelial lining of blood vessels, all in the interstitial zone. The GR was localized in the nuclei of Leydig cells, macrophages and fibroblasts, and endothelial cells lining the blood vessels, all in the interstitium. The immunohistochemical localization of AR, MT1 and GR receptors in the testes of M. terricolor support the previous understanding that testis could be a target for the above-mentioned hormones in this rodent.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2020-09-02

How to Cite

Arora, S., Singh, P., Basu, P., & Haldar, C. (2020). Immunohistochemical Localization of Androgen, Meletonin and Glucocorticoid Receptors in the Testis of Indian Pygmy Field Mouse <i>Mus terricolor</i>. Journal of Endocrinology and Reproduction, 22(2), 31–36. https://doi.org/10.18311/jer/2018/24389

Issue

Volume 22, Issue 2, December 2018

Section

Original Research
Crossref
Scopus
Google Scholar
Europe PMC

 

References

Arora S, Basu P, Singh P, Haldar C. Reproductive physiology of the Indian pygmy field mouse, Mus terricolor. Biol Rhy Res. 2015; 46(1):13-32. https://doi.org/10.1080/092910 16.2014.939443

Arora S. Reproductive physiology of the Indian pygmy field mouse, Mus terricolor [PhD thesis]. Banaras Hindu University; 2013.

Arora S, Singh, P, Basu, P, Haldar C. Effect of exogenous gonadal steroids on reproductive functions of the Indian pygmy field mouse, Mus terricolor. J Endocrinol Reprod. 2014; 18(1):29-36. https://doi.org/10.1080/09291016.2014.939443

Arora S, Singh P, Haldar C. Melatonin in modulation of reproductive functions in the female Indian pygmy field mouse, Mus terricolor. J Scien Res. 2013; 57:27-41.

Arora S, Singh P, Haldar C. Induced stress by glucocorticoid and reproductive function in female Mus terricolor during reproductively active phase. J Endocrinol Reprod. 2012; 16 (2):47-54.

Takeda H, Chodak G, Mutchnik S, Nakamoto T, Chang C. Immunohistochemical localization of androgen receptors with mono- and poly-clonal antibodies to androgen receptor. J Androl. 1990; 126(1):17-25. https://doi.org/10.1677/joe.0.1260017 PMid:2199591

Pelletier G, Labrie C, Labrie F. Localization of oestrogen receptor alpha, oestrogen receptor beta and androgen receptors in the rat reproductive organs. J Endocrinol. 2000; 165(2):35970. https://doi.org/10.1677/joe.0.1650359PMid:10810300

Zhou Q, Clarke L, Nie R, et al. Estrogen action and male fertility: roles of the sodium/hydrogen exchanger-3 and fluid reabsorption in reproductive tract function. Proc Natl Acad Sci. USA. 2001; 98(24):14132-7. https://doi.org/10.1073/ pnas.241245898 PMid:11698654 PMCid:PMC61180

Dubocovich ML, Markowska M. Functional MT1 and MT2 melatonin receptors in mammals. Endocrinology. 2005; 27(2):101-10. https://doi.org/10.1385/ENDO:27:2:101

Vera H, Tijmes M, Valladares LE. Melatonin and testicular function: Characterization of binding sites for 2-[I25I]-iodomelatonin in immature rat testes. Steroids. 1997; 62(2):226-9. https://doi.org/10.1016/S0039-128X(97) 81440-7

Valenti S, Giusti M, Guido R, Giordano G. Melatonin receptors are present in adult rat Leydig cells and are coupled through a pertussis toxin-sensitive G-protein. Eur J Endocrinol. 1997; 136(6):633-9. https://doi.org/10.1530/eje.0.1360633 PMid:9225728

Ahmad R, Haldar C. Effect of intra-testicular melatonin injection on testicular functions, local and general immunity of a tropical rodent Funambulus pennanti. Endocrine. 2010; 37(3):479-488. https://doi.org/10.1007/s12020-0109331-7 PMid:20960172

De Bosscher K, Haegeman G. Minireview: latest perspectives on anti inflammatory actions of glucocorticoids. Mol Endocrinol. 2009; 23(3):281-91. https://doi.org/10.1210/ me.2008-0283 PMid:19095768 PMCid:PMC5428155

Gupta S, Haldar C. Physiological crosstalk between melatonin and glucocorticoid receptor modulates T cell mediated immune responses in a wild tropical rodent, Funambulus pennanti. J Steroid Biochem Mol Biol. 2012; 134:23-36. https://doi.org/10.1016/j.jsbmb.2012.09.013 PMid:23059469

Stalker A, Hermo L, Antakly T. Covalent affinity labeling, radioautography, and immunocytochemistry localize the glucocorticoid receptor in rat testicular Leydig cells. Am J Anat. 1989; 186(4):369-77. https://doi.org/10.1002/ aja.1001860406 PMid:2589221

Schultz R, Isola J, Parvinen M, et al. Localization of the glucocorticoid receptor in testis and accessory sexual organs of male rat. Mol Cell Endocrinol. 1993; 95(1-2):115–20. https://doi.org/10.1016/0303-7207(93)90036-J

Mukherjee A, Haldar C. Photoperiodic regulation of melatonin membrane receptor (MT1R) expression and steroidogenesis in testis of adult golden hamster, Mesocricetus auratus. J Photochem Photobiol B: Biology. 2014; 140:374-80. https://doi.org/10.1016/j.jphotobiol.2014.08.022 PMid:25255424

Ezer N, Robaire B. Androgenic regulation of the structure and functions of the epididymis, In: Robaire B, Hinton B, (Eds.), The Epididymis: From Molecules to Clinical Practice. New York: Kluwer Academic/Plenum Publishers; 2002 p. 297-316. https://doi.org/10.1007/978-1-4615-0679-9_17

Sar M, Lubahn DB, French FS, Wilson EM. Immunohistochemical localization of the androgen receptor in rat and human tissues. Endocrinology. 1990; 127(6):3180-6. https://doi.org/10.1210/endo-127-6-3180 PMid:1701137

Kimura N, Mizokami A, Oonuma T, et al. Immunocytochemical localization of androgen receptor with polyclonal antibody in paraffin-embedded human tissues. J Histochem Cytochem. 1993; 41(5):671-8. https:// doi.org/10.1177/41.5.8468448 PMid:8468448

Bremner WJ, Millar MR, Sharpe RM, Saunders PT. Immunohistochemical localization of androgen receptors in the rat testis: evidence for stage-dependent expression and reglation by androgens. Endocrinology. 1994; 135(3):122734. https://doi.org/10.1210/endo.135.3.8070367 PMid: 8070367

Suarez-Quian CA, Martinez-Garcia F, Nistal M, Regadera J. Androgen receptor distribution in adult human testis. J Clin Endocrinol Metab. 1999; 84(1):350-8. https://doi.org/10.1210/jc.84.1.350

Zhu LJ, Hardy MP, Inigo IV, et al. Effects of androgen on androgen receptor expression in rat testicular and epididymal cells: A quantitative immunohistochemical study. Biol Reprod. 2000; 63(2):368-76. https://doi.org/10.1095/ biolreprod63.2.368 PMid:10906039

Ng TB, Lo L. Inhibitory actions of pineal indoles on steroidogenesis in isolated rat Leydig cells. J Pineal Res. 1988; 5(3):229-43. https://doi.org/10.1111/j.1600079X.1988.tb00649.x PMid:2841443

Most read articles by the same author(s)

1 2 3 > >>