Wound Healing Activity of the Leaves of Lawsonia alba Lam

Abstract

Objective : To screen the wound healing activity of different extracts of leaves of Lawsonia alba on excision, incision wound models in albino rats. Materials and methods : Crude extracts of the leaves of Lawsonia alba obtained by successive solvent extraction with petroleum ether (40-60°C), chloroform, ethanol 95% and finally maceration with chloroform-water, were subjected for phytochemical investigation and were screened for wound healing properties in the excision and incision wound models in albino rats. The flavonoid, luteolin present in the leaves was isolated from ethanol extract by column chromatography. Results : Flavonoids, steroids, tannins and glycosides were found to be present in ethanol extract and other extracts of the leaves of Lawsonia alba. In excision wound parameters the ethanol extract promotes better wound healing (97.28%) compared to control and other extracts. In resutured incision wound models, ethanol extract and petroleum ether extract showed significant Breaking strength (p<0.001) and (p<0.01) respectively compared to control. Conclusion : From the results obtained, it can be observed that ethanol extract of leaves of Lawsonia alba have significant wound healing property. Also it can be concluded that flavonoid may be responsible for wound healing activity.