Formulation, Standardization, and Preclinical Evaluation of Polyherbal Suspension against Inflammatory Bowel Disease

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Authors

  • Prerna Dubey
     IQVIA RDS (India) Private Limited, Bangalore – 560103, Karnataka ,IN
  • Rukaiah Fatma Begum
     Department of Pharmacology, SRM College of Pharmacy, SRMIST, Kattankulathur, Chengalpattu – 603203, Tamil Nadu ,IN
  • V. Chitra
     Department of Pharmacology, SRM College of Pharmacy, SRMIST, Kattankulathur, Chengalpattu – 603203, Tamil Nadu ,IN
  • R. Mrinalini
     Department of Pharmacology, SRM College of Pharmacy, SRMIST, Kattankulathur, Chengalpattu – 603203, Tamil Nadu ,IN ORCID logo http://orcid.org/0000-0002-2228-4636
  • Harini Gunasekaran
     Labcorp Scientific Services and Solutions Private Limited, Mumbai – 400093, Maharashtra ,IN
  • M. Sumithra
     Department of Pharmacology, SRM College of Pharmacy, SRMIST, Kattankulathur, Chengalpattu – 603203, Tamil Nadu ,IN ORCID logo http://orcid.org/0000-0003-1665-0190

DOI:

https://doi.org/10.18311/jnr/2022/29781

Keywords:

Acetic Acid, Inflammatory Bowel Disease, Myeloperoxidase, Polyherbal Suspension, Ulcerative Colitis

Abstract

The pharmacological healing for inflammatory bowel diseases continues to be uncertain and requires immediate therapeutic interventions. A poly-herbal formulation obtained from a traditional and authentic classic text of Ayurveda was assessed for its effect against IBD (inflammatory bowel disease) in this study. The formulated poly-herbal suspension comprises three different drugs namely, Burma dhaniya (Eryngium foetidum), Sapota (Manilkara zapota), and Curry leaves (Murraya koenigii). The formulated suspension was evaluated for certain standard parameters like organoleptic and accelerated stability studies at various temperatures. It was checked for its efficacy by oral route in acetic acid-induced colitis affected Balb/c mice. Mice were orally administered with formulated suspension (275 mg/kg, 550 mg/kg,), every 24 hours for 10 days. Histopathology, macroscopic damage score, myeloperoxidase (MPO) activity, and red blood cell parameters were evaluated after treatment. Reduction in the MPO activity, decrease in the macroscopic damage scores, and an increase in RBC cell count were seen distinctly at a high dose of 550 mg/Kg. The results obtained, established the effectiveness of the poly-herbal suspension against inflammatory bowel disease by treating the mice from acetic acid-induced colitis by reducing inflammation and oxidative damage to the colon. The maximum therapeutic effective activity was found to be 550 mg/kg for IBD mice.

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Published

2022-07-30

How to Cite

Dubey, P., Fatma Begum, R., Chitra, V., Mrinalini, R., Gunasekaran, H., & Sumithra, M. (2022). Formulation, Standardization, and Preclinical Evaluation of Polyherbal Suspension against Inflammatory Bowel Disease. Journal of Natural Remedies, 22(3), 412–423. https://doi.org/10.18311/jnr/2022/29781

Issue

Volume 22, Issue 3, July 2022

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2022-03-11
Accepted 2022-05-17
Published 2022-07-30

 

References

Salminen S, Bouley C, Boutron MC, Cummings JH, Franck A, Gibson GR, et al. Functional food science and gastrointestinal physiology and function. Br J Nutr. 1998; 80(S1):S147–71. https://doi.org/10.1079/BJN19980108. PMid:9849357 DOI: https://doi.org/10.1079/BJN19980108

Riddle MS, Tribble DR, Cachafiero SP, Putnam SD, Hooper TI. Development of a travelers’ diarrhea vaccine for the military: How much is an ounce of prevention really worth?. Vaccine. 2008; 26(20):2490–502. https://doi.org/10.1016/j.vaccine.2008.03.008. PMid:18417259 DOI: https://doi.org/10.1016/j.vaccine.2008.03.008

Loddo I, Romano C. Inflammatory bowel disease: genetics, epigenetics, and pathogenesis. Front Immunol. 2015; 6:551. https://doi.org/10.3389/fimmu.2015.00551. PMid:26579126. PMCid:PMC4629465 DOI: https://doi.org/10.3389/fimmu.2015.00551

Schicho R, Shaykhutdinov R, Ngo J, Nazyrova A, Schneider C, Panaccione R, et al. Quantitative metabolomic profiling of serum, plasma, and urine by 1H NMR spectroscopy discriminates between patients with inflammatory bowel disease and healthy individuals. J. Proteome Res. 2012; 11(6):3344–57. https://doi.org/10.1021/pr300139q. PMid:22574726. PMCid:PMC3558013 DOI: https://doi.org/10.1021/pr300139q

Mu L, Liu L, Niu R, Zhao B, Shi J, Li Y, et al. Indoor air pollution and risk of lung cancer among Chinese female non-smokers. Cancer Causes and Control. 2013; 24(3):439–50. https://doi.org/10.1007/s10552-012-0130-8. PMid:23314675. PMCid:PMC3574203 DOI: https://doi.org/10.1007/s10552-012-0130-8

Vojinovic J. Vitamin D receptor agonists’ anti?inflammatory properties. Ann N Y Acad Sci. 2014; 1317(1):47–56. https://doi.org/10.1111/nyas.12429. PMid:24754474 DOI: https://doi.org/10.1111/nyas.12429

Yamamoto T, Nakahigashi M, Saniabadi AR. Review article: diet and inflammatory bowel disease-epidemiology and treatment. Aliment Pharmacol Ther. 2009; 30(2):99– 112. https://doi.org/10.1111/j.1365-2036.2009.04035.x. PMid:19438426 DOI: https://doi.org/10.1111/j.1365-2036.2009.04035.x

Kaufmann WE, Worley PF, Pegg J, Bremer M, Isakson P. COX-2, a synaptically induced enzyme, is expressed by excitatory neurons at postsynaptic sites in rat cerebral cortex. Proc Natl Acad Sci U.S.A. 1996; 93(6):2317–21. https://doi.org/10.1073/pnas.93.6.2317. PMid:8637870. PMCid:PMC39793 DOI: https://doi.org/10.1073/pnas.93.6.2317

Ricciotti E, FitzGerald GA. Prostaglandins and inflammation. Arterioscler Thromb Vasc Biol. 2011; 31(5):986–1000. https://doi.org/10.1161/ATVBAHA.110.207449. PMid:21508345. PMCid:PMC3081099 DOI: https://doi.org/10.1161/ATVBAHA.110.207449

Izard CE. Four systems for emotion activation: Cognitive and noncognitive processes. Psychol Rev. 1993; 100(1):68. https://doi.org/10.1037/0033-295X.100.1.68. PMid:8426882 DOI: https://doi.org/10.1037/0033-295X.100.1.68

Miller GE, Chen E, Zhou ES. If it goes up, must it come down? Chronic stress and the hypothalamic-pituitary-adrenocortical axis in humans. Psychol Bull. 2007; 133(1):25. https:// doi.org/10.1037/0033-2909.133.1.25. PMid:17201569 DOI: https://doi.org/10.1037/0033-2909.133.1.25

Gowri SS, Vasantha K. Phytochemical screening and antibacterial activity of Syzygium cumini (L.)(Myrtaceae) leaves extracts. Int J Pharm Tech Res. 2010; 2(2):1569–73.

Farnsworth NR. Biological and phytochemical screening of plants. J Pharm Sci. 1966; 55(3):225–76. https://doi.org/10.1002/jps.2600550302. PMid:5335471 DOI: https://doi.org/10.1002/jps.2600550302

Niu X, Fan T, Li W, Huang H, Zhang Y, Xing W. Protective effect of sanguinarine against acetic acid-induced ulcerative colitis in mice. Toxicol Appl Pharmacol. 2013; 267(3):256–65. https://doi.org/10.1016/j.taap.2013.01.009. PMid:23352506 DOI: https://doi.org/10.1016/j.taap.2013.01.009

Krawisz JE, Sharon P, Stenson WF. Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity: Assessment of inflammation in rat and hamster models. Gastroenterology. 1984; 87(6):1344–50. https://doi.org/10.1016/0016-5085(84)90202-6

Tsikas D. Analysis of nitrite and nitrate i biological fluids by assays based on the Griess reaction: Appraisal of the Griess reaction in the l-arginine/nitric oxide area of research. J Chromatogr B. 2007; 851(1–2):51–70. https://doi.org/10.1016/j.jchromb.2006.07.054. PMid:16950667 DOI: https://doi.org/10.1016/j.jchromb.2006.07.054

Masoodi I, Kochhar R, Dutta U, Vaishnavi C, Prasad KK, Vaiphei K, et al. Fecal lactoferrin, myeloperoxidase and serum C-reactive are effective biomarkers in the assessment of disease activity and severity in patients with idiopathic ulcerative colitis. J Gastroenterol Hepatol. 2009; 24(11):1768–74. https://doi.org/10.1111/j.1440-1746.2009.06048.x. PMid:20136960 DOI: https://doi.org/10.1111/j.1440-1746.2009.06048.x

Lih-Brody L, Powell SR, Collier KP, Reddy GM, Cerchia R, Kahn E, et al. Increased oxidative stress and decreased antioxidant defenses in mucosa of inflammatory bowel disease. Dig Dis Sci. 1996; 41:2078–86. https://doi.org/10.1007/BF02093613. PMid:8888724 DOI: https://doi.org/10.1007/BF02093613

Krawisz JE, Sharon P, Stenson WF. Qualitative assay for acute intestinal inflammation based on myeloperoxidase activity. Gastroenterology. 1984; 87:1344–50. https://doi. org/10.1016/0016-5085(84)90202-6 DOI: https://doi.org/10.1016/0016-5085(84)90202-6

Arnhold J. Properties, functions and secretion of human myeloperoxidase. Biochemistry. 2004; 69:4–9. https://doi. org/10.1023/B:BIRY.0000016344.59411.ee. PMid:14972011 DOI: https://doi.org/10.1023/B:BIRY.0000016344.59411.ee

Elson CO, Sartor RB, Tennyson GS, Riddell RH. Experimental models of inflammatory bowel disease. Gastroenterology. 1995; 109:1344–67. https://doi.org/10.1016/0016- 5085(95)90599-5 DOI: https://doi.org/10.1016/0016-5085(95)90599-5

Sharon P, Stenson WF. Metabolism of arachidonic acid in acetic acid colitis in rats. Gastroenterology. 1985; 88:55–63. https://doi.org/10.1016/S0016-5085(85)80132-3 DOI: https://doi.org/10.1016/S0016-5085(85)80132-3

McQuaid KR. Drug used in treatment of gastrointestinal disease. In: Katzung BG, Master SB, Trever AJ, editors. Basic and Clinical Pharmacology. 12th ed. New York: McGraw Hill- Lange; 2012. p. 1081–108.

Billerey- Larmonier C, Uno JK, Larmonier N, Midura AJ, Timmermann B, Ghishan FK, et al. Protective effects of dietary curcumin in mouse model of chemically induced colitis are strain dependent. Inflamm Bowel Dis. 2008; 14(6):780–93. https://doi.org/10.1002/ibd.20348 PMid:18200517. PMCid:PMC4427520 DOI: https://doi.org/10.1002/ibd.20348

Calixto JB. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Braz J Med Biol Res. 2000; 33(2):179–89. https://doi.org/10.1590/S0100-879X2000000200004. PMid:10657057 DOI: https://doi.org/10.1590/S0100-879X2000000200004

Rodrigues TLM, Castro GLS, Viana RG et al. Physiological performance and chemical compositions of the Eryngium foetidum L. (Apiaceae) essential oil cultivated with different fertilizer sources. Nat Prod Res. 2021; 35:5544–8. https://doi.org/10.1080/14786419.2020.1795653. PMid:32691619 DOI: https://doi.org/10.1080/14786419.2020.1795653

Fayek NM, Monem AR, Mossa MY, Meselhy MR, Shazly AH. Chemical and biological study of Manilkara zapota (L.) van Royen leaves (Sapotaceae) cultivated in Egypt. Pharmacognosy Res. 2012; 4:85–91. https://doi.org/10.4103/0974-8490.94723. PMid:22518080. PMCid:PMC3326762 DOI: https://doi.org/10.4103/0974-8490.94723

Srinivasan K. Role of spices beyond food flavoring: Nutraceuticals with multiple health effects. Food Rev Int. 200; 21:167–88. https://doi.org/10.1081/FRI-200051872 DOI: https://doi.org/10.1081/FRI-200051872