Evaluating the Efficacy of a Polyherbal Formulation in Ameliorating Arthritis Induced by Complete Freund’s Adjuvant
Authors
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SRM College of Pharmacy, SRMIST, Kattankulathur, Chennai - 603203, Tamil Nadu ,IN
V. Chitra -
SRM College of Pharmacy, SRMIST, Kattankulathur, Chennai - 603203, Tamil Nadu ,INN. Damodharan
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Department of Pharmacology, Dr Anjali Chatterji Regional Research Institute for Homoeopathy, Kolkata – 700035, West Bengal ,INK. G. V. Narasimha
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SRM College of Pharmacy, SRMIST, Kattankulathur, Chennai - 603203, Tamil Nadu ,INAnil Kumar Yerragopu
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SRM College of Pharmacy, SRMIST, Kattankulathur, Chennai - 603203, Tamil Nadu ,INVara Prasad Saka
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Department of Pharmacology, Sri Padmavathi School of Pharmacy, Tiruchanoor, Tirupati -517503, Andhra Pradesh ,INDhanunjaya Sandopa
DOI:
https://doi.org/10.18311/jnr/2024/34958Keywords:
Arthritis, Formulation, Herbs, Radiographic Analysis, Traditional MedicineAbstract
Background: This study investigates the antiarthritic potential of a polyherbal formulation (PHF) comprising extracts from Tinospora cordifolia, Rosa damescena, and Acacia leucoploea in a rat model of rheumatoid arthritis (RA). RA is a chronic autoimmune disorder with debilitating consequences. PHF's impact on joint inflammation, bone degradation, and cartilage preservation were evaluated. Methods: Arthritis was induced using complete Freund’s adjuvant (CFA), and animals were treated with PHF (200 and 400 mg/kg), prednisolone, or control treatments for 28 days. Parameters including body weight, paw volume, arthritis severity score, hematological parameters, serum markers (creatinine, ALP, total proteins), cytokine levels (IL-1β, IL-6, TNF-α, IL-10), and radiographic changes were assessed. Results: CFA-treated rats exhibited significant body weight loss, paw edema, increased arthritis severity scores, altered hematological parameters, and elevated serum markers compared to normal controls. PHF treatment at both doses mitigated body weight loss, reduced paw edema, and improved arthritis severity scores. Hematological changes induced by CFA were also attenuated by PHF treatment. Serum creatinine, ALP, and total protein levels, elevated in CFA-treated rats, were significantly improved by PHF. Furthermore, PHF modulated cytokine levels, decreasing IL-6, IL-1β, and TNF-α while increasing IL-10. Radiographic analysis displayed reduced joint damage in PHF-treated rats compared to CFA controls. Conclusion: This comprehensive investigation highlights PHF's potential to mitigate the inflammatory processes associated with RA, as evidenced by improved clinical, hematological, and biochemical parameters. The study underscores the promise of traditional botanical compounds in managing RA and suggests PHFs as novel therapeutic options. Further mechanistic studies are warranted to elucidate the exact pathways involved.
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Copyright (c) 2024 V. Chitra, N. Damodharan, K. G. V. Narasimha, Anil Kumar Yerragopu, Vara Prasad Saka, Dhanunjaya Sandopa (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
Accepted 2024-02-14
Published 2024-04-30
References
Alzarea SI, Alasmari AF, Alanazi AS, Alzarea AI, Alharbi M, Alshammari A, et al. Butin attenuates arthritis in completely Freund’s adjuvant-treated arthritic rats: Possibly mediated by its antioxidant and anti-inflammatory actions. Front Pharmacol. 2022; 13(February):1-10. https://doi.org/10.3389/fphar.2022.810052 PMid:35242033 PMCid: PMC8887665
Basile MS, Ciurleo R, Bramanti A, Petralia MC, Fagone P, Nicoletti F, et al. Cognitive decline in rheumatoid arthritis: Insight into the molecular pathogenetic mechanisms. Int J Mol Sci. 2021; 22(3):1185. https://doi.org/10.3390/ ijms22031185 PMid:33530359 PMCid: PMC7865873
Lanzillo CF, Hayden SR. Arthritis, rheumatoid. Rosen and Barkin’s 5-minute emergency medicine consult: Fifth edition. StatPearls Publishing. 2014.
Wagh DS. Arthritis - India. Available at: https://www. arthritis-india.com/rheumatoid-arthritis.html. Accessed August 13, 2023.
Shams S, Martinez JM, Dawson JRD, Flores J, Gabriel M, Garcia G, et al. The therapeutic landscape of rheumatoid arthritis: Current state and future directions. Front Pharmacol. 2021; 12:680043. https://doi.org/10.3389/fphar.2021.680043 PMid:34122106 PMCid: PMC8194305
Alavala S, Nalban N, Sangaraju R, Kuncha M, Jerald MK, Kilari EK, et al. The anti-inflammatory effect of stevioside abates Freund’s Complete Adjuvant (FCA)-induced adjuvant arthritis in rats. Inflammopharmacology. 2020; 28(6):1579-97. https://doi.org/10.1007/s10787-020-00736- 0 PMid:32617791
Zhu L, Chen T, Chang X, Zhou R, Luo F, Liu J, et al. Salidroside ameliorates arthritis-induced brain cognition deficits by regulating the Rho/ROCK/NF-κB pathway. Neuropharmacology. 2016; 103:134-42. https://doi. org/10.1016/j.neuropharm.2015.12.007 PMid:26690894
Alavala S, Nalban N, Sangaraju R, Kuncha M, Jerald MK, Kilari EK, et al. The anti-inflammatory effect of stevioside abates Freund’s Complete Adjuvant (FCA)-induced adjuvant arthritis in rats. Inflammopharmacology. 2020; 28(6):1579-97. https://doi.org/10.1007/s10787-020-00736- 0 PMid:32617791
Chopra A, Lavin P, Patwardhan B, Chitre D. A 32-week randomized, placebo-controlled clinical evaluation of RA-11, an Ayurvedic drug, on osteoarthritis of the knees. JCR J Clin Rheumatol. 2004; 10(5):236-45. https://doi. org/10.1097/01.rhu.0000138087.47382.6d PMid:17043520
Chopra A. Ayurvedic medicine and arthritis. Rheum Dis Clin North Am. 2000; 26(1):133-44. https://doi.org/10.1016/S0889-857X(05)70127-7 PMid:10680201
Petchi R, Parasuraman S, Vijaya C, Gopala KS, Kumar M. Antiarthritic activity of a polyherbal formulation against Freund’s complete adjuvant-induced arthritis in female Wistar rats. J Basic Clin Pharm. 2015; 6(3):77. https://doi. org/10.4103/0976-0105.160738 PMid:26229343 PMCid: PMC4513335
Zhao X, Kim YR, Min Y, Zhao Y, Do K, Son YO. Natural plant extracts and compounds for rheumatoid arthritis therapy. Medicina (B Aires). 2021; 57(3):266. https://doi.org/10.3390/ medicina57030266 PMid:33803959 PMCid: PMC8001474
Zalawadia R, Gandhi C, Patel V, Balaraman R. The protective effect of Tinospora cordifolia on various mast cell- mediated allergic reactions. Pharm Biol. 2009; 47(11):1096-106. https://doi.org/10.3109/13880200903008690
Hajhashemi V, Ghannadi A, Hajiloo M. Analgesic and anti- inflammatory effects of Rosa damascena hydroalcoholic extract and its essential oil in animal models. Iran J Pharm Res IJPR. 2010; 9(2): 163-8.
Qasim S, Alamgeer, Saleem M, Alotaibi NH, Bukhari SNA, Alharbi KS, et al. Appraisal of the antiarthritic potential of prazosin via inhibition of proinflammatory cytokine TNF-α: A key player in rheumatoid arthritis. ACS Omega. 2021; 6(3):2379-88. https://doi.org/10.1021/acsomega.0c05698 PMid:33521476 PMCid: PMC7841939
Zhu L, Zhang Z, Xia N, Zhang W, Wei Y, Huang J, et al. Anti-arthritic activity of ferulic acid in complete Freund’s Adjuvant (CFA)-induced arthritis in rats: JAK2 inhibition. Inflammopharmacology. 2020; 28(2):463-73. https://doi.org/10.1007/s10787-019-00642-0 PMid:31562605
Sannegowda K, Venkatesha S, Moudgil K. Tinospora cordifolia inhibits autoimmune arthritis by regulating key immune mediators of inflammation and bone damage. Int J Immunopathol Pharmacol. 2015; 28(4):521-31. https://doi.org/10.1177/0394632015608248 PMid:26467057 PMCid: PMC8082752
George G, Shyni GL, Mohan S, Abraham B, Nisha P, Ranjith S, et al. In vitro and in vivo anti-inflammatory and anti-arthritic effect of Tinospora cordifolia via modulation of JAK/STAT pathway. Inflammopharmacology. 2023; 31(2):1009-25. https://doi.org/10.1007/s10787-023-01155- 7 PMid:36840884
Sowndhararajan K, Joseph JM, Manian S. Antioxidant and free radical scavenging activities of Indian acacias : Acacia leucophloea (Roxb.) Willd., Acacia ferruginea Dc., Acacia dealbata Link. and Acacia pennata (L.) Willd. Int J Food Prop. 2013; 16(8):1717-29. https://doi.org/10.1080/109429 12.2011.604895
Fontes JA, Barin JG, Talor M V, Stickel N, Schaub J, Rose NR, et al. Complete Freund’s adjuvant induces experimental autoimmune myocarditis by enhancing IL-6 production during initiation of the immune response. Immunity, Inflamm Dis. 2017; 5(2):163-76. https://doi.org/10.1002/iid3.155 PMid:28474508 PMCid: PMC5418134
Patil CR, Rambhade AD, Jadhav RB, Patil KR, Dubey VK, Sonara BM, et al. Modulation of arthritis in rats by Toxicodendron pubescens and its homeopathic dilutions. Homeopathy. 2011; 100(3):131-7. https://doi.org/10.1016/j.homp.2011.01.001 PMid:21784329
Cui X, Wang R, Bian P, Wu Q, Seshadri VDD, Liu L. Evaluation of antiarthritic activity of nimbolide against Freund’s adjuvant-induced arthritis in rats. Artif Cells, Nanomedicine, Biotechnol. 2019; 47(1):3391-8. https://doi.org/10.1080/21691401.2019.1649269 PMid:31394949
Mossiat C, Laroche D, Prati C, Pozzo T, Demougeot C, Marie C. Association between arthritis score at the onset of the disease and long-term locomotor outcome in adjuvant-induced arthritis in rats. Arthritis Res Ther. 2015; 17(1):184. https://doi.org/10.1186/s13075-015-0700-8 PMid:26183428 PMCid: PMC4506462
Kshirsagar AD, Panchal PV, Harle UN, Nanda RK, Shaikh HM. Anti-inflammatory and antiarthritic activity of anthraquinone derivatives in rodents. Int J Inflam. 2014; 2014:1-12. https://doi.org/10.1155/2014/690596 PMid:25610704 PMCid: PMC4290027
Petchi RR, Vijaya C, Parasuraman S. Anti-arthritic activity of ethanolic extract of Tridax procumbens (Linn.) in Sprague Dawley rats. Pharmacognosy Res. 2013; 5(2):113-7. https://doi.org/10.4103/0974-8490.110541 PMid:23798886 PMCid: PMC3685759
Sharma B, Kumar P. Extraction and pharmacological evaluation of some extracts of Tridax procumbens and Capparis decidua. Int J Appl Res Nat Prod. 2008; 1(4):5-12.
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