A Validated HPTLC Method for Quantification of Linoleic Acid and Beta-Sitosterol in Solanum nigrum Extract

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Authors

  • Arpan Chakraborty
     Department of Pharmaceutical Technology, Maulana Abul Kalam Azad University of Technology, Nadia - 741249, West Bengal, ,IN
  • Arka Bhattacharjee
     Department of Pharmaceutical Technology, Maulana Abul Kalam Azad University of Technology, Nadia - 741249, West Bengal ,IN
  • Baishakhi Mondal
     Department of Pharmaceutical Technology, Maulana Abul Kalam Azad University of Technology, Nadia - 741249, West Bengal ,IN
  • Manas Chakraborty
     Department of Pharmaceutical Technology, Calcutta Institute of Pharmaceutical Technology and Allied Health Sciences, Howrah - 711316, West Bengal ,IN
  • Goutam Mukhopadhyay
     Department of Pharmaceutical Technology, BCDA College of Pharmacy and Technology (Campus – 2), Kolkata - 700129, West Bengal ,IN
  • Alpana Majumder
     Department of Kayachikitsa, Institute of Post Graduate Ayurvedic Education and Research, Kolkata - 700009, West Bengal ,IN
  • Maitrish Ghosh
     Department of Pharmaceutical Technology, NSHM College of Pharmaceutical Technology, Kolkata - 700053, West Bengal ,IN

DOI:

https://doi.org/10.18311/jnr/2023/33964

Keywords:

β-sitosterol, HPTLC, Linoleic Acid, Solanum nigrum, Validation

Abstract

Solanum nigrum, a member of the Solanaceae family, has long been used as both food and medicine. This plant has been recognized for its immunomodulatory, anti-ulcerogenic, hepatoprotective, and anti-hyperlipidemic effects. It contains various chemical components such as alkaloids, coumarins, flavonoids, tannins, saponins, proteins, carbohydrates, glycosides, and phytosterols. The objective of the current work was to create a repeatable and consistent HPTLC-validated technique for concurrently detecting Beta-Sitosterol (BTS) and Linoleic Acid (LIA) in S. nigrum in accordance with ICH recommendations. The HPTLC process involved using silica gel 60 F254 TLC plates as the stationary phase and a mobile phase consisting of n-hexane, ethyl acetate, and formic acid in a ratio of 6:4:0.2 (v/v/v). The resulting bands were uniformly visualized after derivatization with anisaldehyde sulfuric acid. The calibration curves of standard LIA and BTS exhibited satisfactory linearity within concentration ranges of 400-1150 ng/spot and 350-1200 ng/spot, respectively, with correlation coefficients (r2) of 0.99153 and 0.99389. LOD and LOQ were determined as 91.96 and 118.14 ng/spot for LIA, and 278.66 and 314.62 ng/spot for BTS, respectively. In conclusion, this HPTLC method proved to be efficient, simple, precise, and reproducible for measuring LIA and BTS in S. nigrum.

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Published

2023-08-31

How to Cite

Chakraborty, A., Bhattacharjee, A., Mondal, B., Chakraborty, M., Mukhopadhyay, G., Majumder, A., & Ghosh, M. (2023). A Validated HPTLC Method for Quantification of Linoleic Acid and Beta-Sitosterol in <i>Solanum nigrum</i> Extract. Journal of Natural Remedies, 23(3), 871–878. https://doi.org/10.18311/jnr/2023/33964

Issue

Volume 23 Issue 3 July 2023

Section

Research Articles

License

Copyright (c) 2023 Arpan Chakraborty, Arka Bhattacharjee, Baishakhi Mondal, Manas Chakraborty, Goutam Mukhopadhyay, Alpana Majumder, Maitrish Ghosh (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Received 2023-06-04
Accepted 2023-07-24
Published 2023-08-31

 

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