An Improved HPLC Method for Estimation of Phyllanthin and Hypophyllanthin in <I>Phyllanthus amarus</I>

B. Murali; A. Amit; M. S. Anand; T. K. Dinesh; D. S. Samiulla

doi:10.18311/jnr/2001/63

An Improved HPLC Method for Estimation of Phyllanthin and Hypophyllanthin in Phyllanthus amarus

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Authors

B. Murali
,IN
A. Amit
,IN
M. S. Anand
,IN
T. K. Dinesh
,IN
D. S. Samiulla
,IN

DOI:

https://doi.org/10.18311/jnr/2001/63

Keywords:

Phyllanthus amarus, Phyllanthin, Hypophyllanthin, HPLC.

Therapeutic Drug Monitoring

Abstract

Objective: To develop an improved HPLC method for estimation of phyllanthin and hypophyllanthin in Phyllanthus amarus. Materials and methods: An Isocratic, reversed phase (RP) HPLC procedure has been adopted using a mixture of pH 2.8 Phosphate buffer and acetonitrile as mobile phase, CN column as stationary phase and UV detector. Results: The developed method shows high resolution (R = 1.9), accuracy and reproducibility. Conclusion: The method developed is relatively better in terms of separation than the previously reported methods.

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Published

2001-01-01

How to Cite

Murali, B., Amit, A., Anand, M. S., Dinesh, T. K., & Samiulla, D. S. (2001). An Improved HPLC Method for Estimation of Phyllanthin and Hypophyllanthin in <I>Phyllanthus amarus</I>. Journal of Natural Remedies, 1(1), 55–59. https://doi.org/10.18311/jnr/2001/63