Effect of Thyponium flageliforme in Combination with Curcuma zedoaria and Phyllanthus niruri in Expression of Protein Estrogen Receptor and Caspase-9 on Breast Cancer Mice
Authors
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Department of Chemistry, Medical Faculty, Sultan Agung Islamic University, Jawa Tengah - 50112 ,ID
Eni Widayati -
Department of Anatomy, Medical Faculty, Sultan Agung Islamic University, Jawa Tengah - 50112 ,IDChodidjah
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Department of Biochemistry, Sultan Agung Islamic University, Semarang, Jawa Tengah - 50112 ,IDTaufiqurrachman Nasihun
DOI:
https://doi.org/10.18311/jnr/2018/8539Keywords:
Breast Cancer, Caspase9, Combination of Herbal Extracts, Estrogen ReceptorsAbstract
Introduction: Incidence of breast cancer is high and constitutes a significant cause of female mortality. One of breast cancer type is Estrogen and Progesteron Receptor (ER and PR) positive, therefore affected by estrogen and progesteron concentration. Treatment of Thyponium flageliforme in combination with Curcuma zedoaria, and Phyllanthus niruri (TCP) have been proven capable of inhibiting tumor cells proliferation. Objective: To evaluate the expression of protein estrogen receptor and caspase-9 in C3H mice were inoculated with adenocarcinoma cells. Methods: 18 mice were inoculated with one million cells of breast adenocarcinoma per mouse and assigned into three groups. TCP with dosage 85 and 125 mg were treated for 21 days to evaluate the expression of protein estrogen receptor and caspase-9 measured by immuno-histochemical staining. Results: Mann Whitney analysis demonstrated that expression of protein estrogen receptor and caspase-9 in TCP 125 was significant (p < 0.05). Conclusion: Expression of protein estrogen receptor was decreased and caspase-9 expression was increased by TCP administration.
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Accepted 2018-04-17
Published 2018-05-09
References
DeSantis CE, Bray F, Ferlay J, Tieulent JL, Anderson BO, Jemal A. International variation in female breast cancer incidence and mortality rates. Cancer Epidemiology, Biomarkers and Prevention. 2015; 24(10):1495–506. Crossref. PMid:26359465
Ferlay J, Soerjomataram I, Ervik M, et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2015; 136(5):E359-86. Crossref. PMid:25220842
Pusat Data dan Informasi Kementerian Kesehatan RI. Situasi Penyakitkanker. Buletin Jendela Data dan Informasi Kesehatan. Semester 1, 2015.
Strumfa I, Vanags A, Abolins A, Gardovskis J. Pathology of breast cancer: From classic concepts to molecular pathology and pathogenesis. Acta Chirurgica Latviensis. 2012; 12(1):59–66. Crossref.
Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T, Thun MJ. Cancer statistics, 2008, CA. Cancer J Clin. 2008; 58(2):71–96. Crossref. PMid:18287387
Bombonati A, Sgroi DC. The molecular pathology of breast cancer progression. J Pathol. 2011; 223(2):307–17. Crossref. PMid:21125683 PMCid:PMC3069504
Berry DA, Cronin KA, Plevritis SK, et al. Effect of screening and adjuvant therapy on mortality from breast cancer. N Engl J Med. 2005; 353(17):1784–92. Crossref. PMid:16251534
Brenner H, Stegmaier C, Ziegler H. Recent improvement in survival of breast cancer patients in Saarland, Germany. British Journal of Cancer. 1998; 78(5):694–7. Crossref. PMid:9744513 PMCid:PMC2063047
Chodidjah C, Widayati E, Nasihun T. Treatment of Thyponium flageliforme in combination with Curcuma zedoaria, and Phyllanthus niruri synergistically enhances apoptotic and anti-proliferative effect on breast cancer. Journal of Natural Remedies. 2017; 17(1):1–8. Crossref.
Wambi JSL, Jordan VC. Estrogen regulation of apoptosis: How can one hormone stimulate and inhibit? Breast Cancer Research. 2009; 11(3):1–12.
Ghartey Jnr FN, Anyanful A, Eliason S, Adamu SM, Debrah S. Pattern of breast cancer distribution in ghana: A survey to enhance early detection, diagnosis, and treatment. International Journal of Breast Cancer. 2016; 3645308:1–9. Crossref. PMCid:PMC5007313
Schacter JL, Henson ES, Gibson SB. Estrogen regulation of anti-apoptotic bcl-2 family member mcl-1 expression in breast cancer cells. PLoS ONE. 2014; 9(6):e100364. Crossref. PMid:24971890 PMCid:PMC4074091
Cui J, Shen Y, Li R. Estrogen synthesis and signaling pathways during aging: From periphery to brain. Trends in Molecular Medicine. 2013; 19(3):197–209. Crossref. PMCid:PMC3595330
Brisken C, O'Malley B. Hormone action in the mammary gland. Cold Spring Harb Perspect Biol. 2010; 2(12): a003178. Crossref. PMid:20739412 PMCid:PMC2982168
Lerner LJ, Jordan VC. The development of antiestrogens for the treatment of breast cancer. Cancer Res. 1990; 50(14):4177–89. PMid:2194650
BeatsonGT. On the treatment of inoperable cases of carcinoma of the mamma: suggestions for a new method of treatment with illustrative cases. Lancet. 1896; 148(2):162–5. Crossref.
Farvid MS, Eliassen AH, Cho E, Liao X, Chen WY, Willett WC. Dietary fiber intake in young adults and breast cancer risk. Pediatrics. 2016; 137(3):e20151226. Crossref. PMid:26908709 PMCid:PMC4771124
Shi Y. Mechanisms of caspase activation and inhibition during apoptosis. Mol Cell. 2002; 9(3):459–70. Crossref.
Hu Q, Wu D, Chen W, Yan Z, Shi Y. Proteolytic processing of the caspase-9 zymogen is required for apoptosomemediated activation of caspase-9. The Journal of Biological Chemistry. 2013; 288(21):5142–7. Crossref. PMid:23572523 PMCid:PMC3663534
Lee SH, Jaganath, Wang SM, Sekaran SD. Antimetastatic effect of Phyllantuhs in human lung (A 549) and breast (MCF-7) cancer cell lines. PLoS ONE; 2011; 6(6):e20994. Crossref. PMid:21698198 PMCid:PMC3116853
Lakshmi S. Padmaja G, Remani P. Antitumour effects of isocurcumenol isolated from curcuma zedoaria rhizomes on human andmurine cancer cells. International Journal of Medicinal Chemistry. 2011; 253962:1–13. Crossref. PMid:27429805 PMCid:PMC4939266
Rahman A S M, Wahab AN, Malek ASN. In vitro morphological assessment of apoptosis induced by anti proliferative constituents from the rhizomes of Curcuma zedoaria. Evidence-Based Complementary and Alternative Medicine. 2013; 257108:1–14. Crossref. PMid:23762112 PMCid:PMC3671673
Wang LK, Lin-Shiau SY, Lin JK. Induction of apoptosis by apigenin and related flavonoids through cytochrome c release and activation of caspase-9 and caspase-3 in leukaemia HL-60 cells. European Journal of Cancer. 1999; 35(10):1517–25. Crossref.
Papademetrio DL, Trabucchi A, Cavaliere V, et al. The Cathechin flavonoid reduced proliferation and induced apoptosis of murine lymphoma cells LB02 through modulation of antiapoptotic proteins. Revista Brasileira de Farmacognosia. 2013; 23(3):455–63. Crossref.
Widayati E, Nasihun T. Treatment of Pimpinella Alpina Molk improve oxidative stress and inhibit liver cellular apoptosis in rats following UVB irradiation: Is there any correlation? Bangladesh Journal of Medical Science. 2018; 17(1):118–28. Crossref.
Fuida S, Debatin KM. Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy. Oncogene. 2006; 25(34):4798–811. Crossref. PMid:16892092
Lakshmi S, Padmaja G, Remani P. Antitumoureffects of isocurcumenol isolated from Curcuma zedoaria rhizomes on human and murine cancer cells. International Journal of Medicinal Chemistry. 201; 253962:1–13.
Arpino G, Wiechmann L, Osborne CK, Schiff R. Crosstalk between the estrogen receptor and the HER tyrosine kinase receptor family: molecular mechanism and clinical implications for endocrine therapy resistance. Endocr Rev. 2008; 29(2):217–33. Crossref. PMid:18216219 PMCid: PMC2528847
Casa AJ, Potter AS, Malik Set al. Estrogen and insulinlike growth factor-I (IGF-I) independently down-regulate critical repressors of breast cancer growth. Breast Cancer Res Treat. 2012; 132(1):61–73. Crossref. PMid:21541704 PMCid:PMC3936881
Larocca LM, Teofili L, Maggiano N, Piantelli M, Ranelletti FO, Leone G. Quercetin and the growth of leukemic progenitors. Leuk Lymphoma. 1996; 23(1-2):49–53. Crossref. PMid:9021685
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