Development and Validation of HPTLC Method for Simultaneous Estimation of Piperine and Scopoletin in Ajmodadi churna
Authors
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Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia - 391760, Vadodara, Gujarat. ,IN
Tanvi Dodiya -
Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia - 391760, Vadodara, Gujarat. ,INNisha Somavanshi
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Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia - 391760, Vadodara, Gujarat. ,INDisha Prajapati
DOI:
https://doi.org/10.18311/jnr/2023/31349Keywords:
Ajmodadi Churna, HPTLC, Piperine, Scopoletin.Abstract
In this recent study, several trials were made to develop a HPTLC method for quantification of scopoletin and piperine in the Ajmodadi formulation. HPTLC was done on pre-coated silica gel 60 F254 plates with a mobile phase of toluene:ethyl acetate:methanol:formic acid (3.9:3.9:0.3:1.7 v/v/v/v). The retardation factors for scopoletin 0.75 and for piperine was 0.86 and found a good and defined resolution peak. Densitometer analysis of scopoletin and piperine was carried out at 335 wavelength (nm). The developed method was validated as reported in ICH guidelines (Q2R1). A linearity study shows that scopoletin and piperine was linear, and the recovery studies revealed a recovery in between 98-102 % as per guideline. This method was established to be rapid, delicate, accurate and specific, thus, these methods may be used in the quantification of the drug in any polyherbal formulation.
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Copyright (c) 2023 Tanvi Tanvi, Nisha Somavanshi, Disha Prajapati (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
Accepted 2023-03-23
Published 2023-03-23
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