Method Development and Validation of HPTLC Method for Simultaneous Estimation of Atropine, Piperine and Vasicine in Ayurvedic Formulation

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Authors

  • Sanjay Yadav
     Department of Quality Assurance, Parul Institute of Pharmacy and Research, Parul University Limda, Vadodara - 391760, Gujarat ,IN
  • Tanvi Dodiya
     Department of Pharmacognosy, Parul Institute of Pharmacy and Research, Parul University Limda, Vadodara - 391760, Gujarat ,IN
  • Disha Prajapati
     Department of Pharmacognosy, Parul Institute of Pharmacy and Research, Parul University Limda, Vadodara - 391760, Gujarat ,IN
  • Jaydeep Patil
     Department of Quality Assurance, Parul Institute of Pharmacy and Research, Parul University Limda, Vadodara - 391760, Gujarat ,IN
  • Mansi Patel
     Department of Quality Assurance, Parul Institute of Pharmacy and Research, Parul University Limda, Vadodara - 391760, Gujarat ,IN

DOI:

https://doi.org/10.18311/jnr/2024/41922

Keywords:

Atropine, HPTLC, Piperine, Vasicine

Abstract

Background: Dhumayoga, an Ayurvedic formulation is used for the management of respiratory disorders, including dyspnea and asthma. Aim: To develop and validate a High-Performance Thin-Layer Chromatography (HPTLC) method for the simultaneous estimation of atropine, vaccine and piperine in Dhumayoga Ayurvedic formulation. Methods: The three biomarkers were resolved using precoated silica gel 60F254 as stationary phase, dioxane: toluene: methanol: ethyl acetate: Aqueous ammonia (4:7:3:5:1v/v/v/v/v) as mobile phase and detected and quantified at 254nm. Results: The Rf values of atropine, vasicine, and piperine were found to be 0.406 ± 0.02, 0.56 ± 0.02, 0.87 ± 0.02, respectively. The calibration plot was linear in the range of 500-2500ng per zone with a correlation coefficient (r2) of atropine, vasicine and piperine was 0.994, 0.9986 and 0.9935, respectively. Conclusion: The developed method was applied for the quantitation of biomarkers in Dhumayoga. The method was validated in terms of linearity, precision, specificity, accuracy and robustness as per International Council for Harmonisation (ICH) guidelines.

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Published

2024-11-06

How to Cite

Yadav, S., Dodiya, T., Prajapati, D., Patil, J., & Patel, M. (2024). Method Development and Validation of HPTLC Method for Simultaneous Estimation of Atropine, Piperine and Vasicine in <i>Ayurvedic</i> Formulation. Journal of Natural Remedies, 24(10), 2207–2215. https://doi.org/10.18311/jnr/2024/41922

Issue

Volume 24, Issue 10, October 2024

Section

Research Articles

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Copyright (c) 2024 Sanjay Yadav, Tanvi Dodiya, Disha Prajapati, Jaydeep Patil, Mansi Patel (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Received 2024-03-04
Accepted 2024-07-05
Published 2024-11-06

 

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