Cytotoxic Activity of Mangosteen (Garcinia mangostana L.) Peel Extract and α-mangostin toward Leukemia Cell Lines (HL-60 and K-562)
Authors
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School of Health Sciences Jenderal Achmad Yani, Cimahi ,ID
Arina Novilla -
School of Health Sciences Jenderal Achmad Yani, Cimahi ,IDDedi S. Djamhuri
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Biomedical and Biomolecular Research Centre, Aretha Medika Utama, Bandung, Indonesia ,IDNurul Fauziah
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Biomedical and Biomolecular Research Centre, Aretha Medika Utama, Bandung ,IDMaesaroh Maesaroh
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Biomedical and Biomolecular Research Centre, Aretha Medika Utama, Bandung ,IDBalqis Balqis
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Faculty of Medicine, Maranatha Christian University, Bandung ,IDWahyu Widowati
DOI:
https://doi.org/10.18311/jnr/2016/842Keywords:
Mangosteen Peel Extract, α, -mangostin, acute myeloid leukemia (AML), chronic myeloid leukemia (CML), selectivity indexAbstract
Fruit of Mangosteen (Garcinia mangostana L.) is well-known in Indonesia and other Southeast Asian countries. Studies have shown that extract of the pericarp of mangosteen contained mostly of xanthones exhibit many biological activities, especially as an antitumor. This study aimed to investigate the cytotoxic activity and selectivity of Mangosteen Peel Extract (MPE) and α-mangostin against two leukemia cell lines (HL-60 and K-562) and the normal lymphocyte cells from two different donors. The cytotoxic activity was performed using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxyme-thoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Imatinib and Isotretinoin were used as a positive control to the K-562 and HL-60 cells, respectively. The MPE and α-mangostin revealed higher mortality toward leukemia cell lines rather than toward lymphocyte cells, with more than 80% of HL-60 and K-562 cells died at 6.25 and 25 μg/ml, respectively. MPE was more toxic and selective against K-562 with IC50 of 2.79 μg/ml and SI of 8.27, while α-mangostin was more toxic and selective against HL-60 with IC50 of 1.12 μg/ml and SI of 22.34. MPE and α-mangostin showed potent sensitivity and selectivity to leukemia cells, hence these are considered as promising sources for future leukemia treatment.
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Accepted 2016-04-13
Published 2016-08-18
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