Evaluation of Aldrin-Induced Oxidative Stress and Apoptosis in J774 Macrophages

Abstract

The present study was carried out to evaluate the aldrin-induced oxidative stress and apoptosis in murine macrophage (J774A.1) cells. Acute exposure of aldrin for 2 hrs was given to the J774A.1 cells cultured in DMEM with 10% FBS at 37oC and 5% CO2 in air with 95% relative humidity under in vitro system. Lethal concentration-50 (LC50) was calculated after exposure period as 7.24 μg/ml. Further, cells were exposed with three different concentration of aldrin (1.81, 3.65 and 7.24 μg/ml) and 0.1% DMSO was used as negative control. The antioxidant enzymes and non-enzymes were determined along with G6pDH, LDH and ALP enzymes in J774A.1 cells. Cells were monitored for cell morphology and apoptosis. Exposure of aldrin to J774A.1cells resulted in increase in lipid peroxidation and decrease in antioxidant enzyme/ nonenzymes system. Further it caused decrease in G6PDH enzymes activity and excess leakage of LDH and ALP enzymes. The aldrin-treated cells showed higher number of apoptotic cells with alteration in cell morphology indicating apoptotic and necrotic changes. These effects were noticed in dose dependant manner. In conclusion, the result of in vitro study suggests that the aldrin can induced the process of apoptosis and cell death through the generation of ROS and thereof oxidative insult in J774A.1 cells.