DNA Hypomethylation could be a Predictive Biomarker for B Vitamin Deficiencies Among Working Population
Authors
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Department of Biochemistry, Regional Occupational Health Centre (Southern), Indian Council of Medical Research, Bengaluru – 562110, Karnataka ,IN
Kalahasthi Ravi Babu -
Department of Biochemistry, Regional Occupational Health Centre (Southern), Indian Council of Medical Research, Bengaluru – 562110, Karnataka ,INVinay Kumar Adepu
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Department of Biochemistry, Regional Occupational Health Centre (Southern), Indian Council of Medical Research, Bengaluru – 562110, Karnataka ,INRaju Nagaraju
DOI:
https://doi.org/10.18311/ti/2024/v31i3/42028Keywords:
B Vitamins, Diagnostic Accuracy, DNA Methylation, 5-MethylcytosineAbstract
The methylation (CH3) capacity is dependent on the availability of B vitamins in one carbon metabolism. This study evaluated B vitamin (B9 and B12) deficiency status and its impact on the Diagnostic Accuracy (DA) of DNA methylation among the working population. DNA methylation (5 methylcytosine) and B vitamins were quantified using the ELISA methods. Based on the cut-off values of B vitamins, the subjects were categorized into adequate, marginal, and deficiency status. Fagan’s nomogram was used to assess the DA of DNA methylation in different conditions of B vitamin. DA metrics are used in this study as a Diagnostic Odd Ratio (DOR), a positive Likelihood Ratio (LR+), a negative Likelihood Ratio (LR-), and a posttest probability of true positive DNA methylation that is less than 2.0%. DNA methylation was significantly lower in marginal and deficient B-vitamin status as compared to adequate status. The DA metrics at 5 mC showed high sensitivity and modest specificity for marginal and deficient B vitamins. In vitamin B9 deficiency, LR (+) was 2.51 (95% CI: 0.99–6.39), DOR was 10.4 (95% CI: 2.06–51.2), and the post-test probability was 92% (95% CI: 83–97). In vitamin B12 deficiency, LR (+) was 1.58 (95% CI: 1.03–2.44), DOR was 3.16 (95% CI: 1.29–7.66), and the post-test probability was 86% (95% CI: 80–90). In B vitamin deficiency, DA metrics of DNA methylation: LR+, LR-, DOR, and post-test probability were more precisely predicted. DNA hypomethylation could be used as a predictive biomarker in B vitamin deficiency. The findings of the study help to prevent adverse health effects due to methylation capacity loss.
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Copyright (c) 2024 Kalahasthi Ravi Babu, Vinay Kumar Adepu, Raju Nagaraju
This work is licensed under a Creative Commons Attribution 4.0 International License.
Accepted 2024-04-15
Published 2024-08-05
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