Biochemical Profiling and Chemopreventive Activity of Phloretin on 7,12"‘Dimethylbenz (A) Anthracene Induced Oral Carcinogenesis in Male Golden Syrian Hamsters

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Authors

  • M. Arokia Vijaya Anand
     ,IN
  • K. Suresh
     ,IN

Keywords:

Antioxidant, chemoprevention, 7, 12-dimethylbenz (a) anthracene, lipid peroxidation, oral cancer, phloretin

Abstract

Objectives: The present study was designed to examine the chemopreventive effects of phloretin against 7, 12"‘dimethylbenz (a) anthracene (DMBA) induced buccal pouch carcinogenesis in male golden Syrian hamsters in order to discover resources to improve the traditional medicine. Materials and Methods: Hamsters were divided into four groups of 10 animals each. Group I was served as an untreated control. Group II hamsters were painted with 0.5% DMBA in liquid paraffin on the left buccal pouches three times a week for 14 weeks. Group III hamsters were orally administrated with phloretin at a dose of 40 mg/kg body Weight (b.wt) on days alternate to DMBA application. Group IV hamsters were orally administrated with phloretin alone and served as the drug control. The experiment was terminated at the end of fourteenth week. The experimental animal's tumors were subjected into morphological examination and subsequently screened the pathological changes and estimate the activities of bi"‘products of lipid peroxidation, antioxidants enzymes and phase I and II detoxification enzyme status. Results: In DMBA alone treated hamster showed increased levels of lipid peroxidation by products, leads to decreased levels of enzymatic and non"‘enzymatic antioxidants status, activities of phase I and II detoxification enzyme status were altered. Normalized the neoplastic changes, decreased the levels of lipid by products, retain the antioxidants and restored the phase I and II enzymes were observed in phloretin administrated animals during DMBA induced oral carcinogenesis. Conclusion: Phloretin has possible chemopreventive role in which modulating the antioxidant and detoxification enzyme status, thereby retarding DMBA induced buccal pouch carcinogenesis.

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Published

2018-04-26

How to Cite

Arokia Vijaya Anand, M., & Suresh, K. (2018). Biochemical Profiling and Chemopreventive Activity of Phloretin on 7,12"‘Dimethylbenz (A) Anthracene Induced Oral Carcinogenesis in Male Golden Syrian Hamsters. Toxicology International, 21(2), 179–185. Retrieved from https://informaticsjournals.co.in/index.php/toxi/article/view/21002

Issue

Volume 21, Issue 2, May-Aug 2014

Section

Original Research
Received 2018-04-26
Accepted 2018-04-26
Published 2018-04-26

 

References

World Health Organization Databank. WHO Statistical Information System. Geneva: World Health Organization 2010. Available from: http://www.who.int/whosis [Last accessed on 2014 Jan 22].

Coelho KR. Challenges of the oral cancer burden in India. J Cancer Epidemiol 2012;2012:701932.

Nair U, Bartsch H, Nair J. Alert for an epidemic of oral cancer due to use of the betel quid substitutes gutkha and pan masala: A review of agents and causative mechanisms. Mutagenesis 2004;19:251"‘62.

Halliwell B, Whiteman M. Measuring reactive species and oxidative damage in vivo and in cell culture: How should you do it and what do the results mean?. Br J Pharmacol 2004;142:231"‘55.

Das SK. Free radicals, antioxidants and nutraceuticals in health, disease and radiation biology. Preface. Indian J Biochem Biophys 2012;49:291"‘2.

Forman HJ, Davies KJ, Ursini F. How do nutritional antioxidants really work: Nucleophilic tone and para"‘hormesis versus free radical scavenging in vivo. Free Radic Biol Med 2014;66:24"‘35.

Middleton E Jr, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacol Rev 2000;52:673"‘751.

Kern M, Pahlke G, Balavenkatraman KK, Bohmer FD, Marko D. Apple polyphenols affect protein kinase C activity and the onset of apoptosis in human colon carcinoma cells. J Agric Food Chem 2007;55:4999"‘5006.

Kobori M, Iwashita K, Shinmoto H, Tsushida T. Phloretin"‘induced apoptosis in B16 melanoma 4A5 cells and HL60 human leukemia cells. Biosci Biotechnol Biochem 1999;63:719"‘25.

Geren RJ, Greenberg NH, Mcdonald MM, Schumacher AM. Protocols for screening chemical agents and natural products against animal tumors and other biological systems. Cancer Chemother Rep 1972;3:1"‘103.

Ray RS, Ghosh B, Rana A, Chatterjee M. Suppression of cell proliferation, induction of apoptosis and cell cycle arrest: Chemopreventive activity of vanadium in vivo and in vitro. Int J Cancer 2006;120:13"‘23.

Dodge JF, Mitchell G, Hanahan DJ. The preparation and chemical characterization of hemoglobin"‘free ghosts of human red blood cells. Arch Biochem Biophys 1968;110:119"‘30.

Quist EE. Regulation of erythrocycaste membrane shape by Ca2+. Biochem Biophys Res Commun 1980;92:631"‘7.

Yagi K. Lipid peroxides and human diseases. Chem Phys Lipids 1987;45:337"‘51.

Jiang ZY, Hunt JV, Wolff SP. Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low density lipoprotein. Anal Biochem 1992;202:384"‘9.

Rao KS, Recknagel RO. Early onset of lipoperoxidation in rat liver after carbon tetrachloride administration. Exp Mol Pathol 1968;9:271"‘8.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984;21:130"‘2.

Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389"‘94.

Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: Biochemical role as a component of glutathione peroxidise. Science 1973;179:588"‘90.

Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61:882"‘8.

Omaye ST, Turnbull TD, Sauberlich HE. Selected method for the determination of ascorbic acid in animal cells, tissues and fluids. Methods Enzymol 1979;62:3"‘11.

Desai ID. Vitamin E analysis methods for animal tissues. Methods Enzymol 1984;105:138"‘47.

Omura T, Sato R. The carbon monoxide"‘binding pigment of liver microsomes. II. solubilization, purification, and properties. J Biol Chem 1964;239:2379"‘85.

Carlberg I, Mannervik B. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. J Biol Chem 1975;250:5475"‘80.

Habig WH, Pabst MJ, Fleischner G, Gatmaitan Z, Arias IM, Jakoby WB. The identity of glutathione S"‘transferase B with ligandin, a major binding protein of liver. Proc Natl Acad Sci U S A 1974;71:3879"‘82.

Fiala S, Fiala AE, Dixon B. Glutamyl transpeptidase in transplantable, chemically induced rat hepatomas and "spontaneous” mouse hepatomas. J Natl Cancer Inst 1972;48:1393"‘401.

Casto BC, Knobloch TJ, Galioto RL, Yu Z, Accurso BT, Warner BM. Chemoprevention of oral cancer by lyophilized strawberries. Anticancer Res 2013;33:4757"‘66.

Chiang CP, Huang WT, Lee JW, Hsu YC. Effective treatment of 7,12"‘dimethylbenz (a) anthracene induced hamster buccal pouch precancerous lesions by topical photosan mediated photodynamic therapy. Head Neck 2012;34:505"‘12.

Yang K, Zhang G, Mei J, Chen D, Wu M. Screening and analysis of pathogenic genes during DMBA induced buccal mucosa carcinogenesis in golden hamsters. Oncol Rep 2010;23:1619"‘24.

Rajalingam K, Sugunadevi G, Arokia Vijayaanand M, Kalaimathi J, Suresh K. Anti"‘tumour and anti"‘oxidative potential of diosgenin against 7, 12"‘dimethylbenz (a) anthracene induced experimental oral carcinogenesis. Pathol Oncol Res 2012;18:405"‘12.

Tania M, Khan MA, Song Y. Association of lipid metabolism with ovarian cancer. Curr Oncol 2010;17:6"‘11.

Suresh K, Manoharan S, Vijayaanand MA, Sugunadevi G. Chemopreventive and antioxidant efficacy of (6)"‘paradol in 7,12"‘dimethylbenz (a) anthracene induced hamster buccal pouch carcinogenesis. Pharmacol Rep 2010;62:1178"‘85.

Thirunavukkarasu C, Sakthisekaran D. Effect of selenium on Nnitrosodiethylamine induced multi stage hepatocarcinogenesis with reference to lipid peroxidation and enzymic antioxidants. Cell Biochem Funct 2001;19:27"‘35.

Samy RP, Gopalakrishnakone P, Ignacimuthu S. Anti"‘tumor promoting potential of luteolin against 7,12"‘dimethylbenz (a) anthracene"‘induced mammary tumors in rats. Chem Biol Interact 2006;164:1"‘14.

Sies H. Strategies of antioxidant defense. Eur J Bichem 1993;215:213"‘9.

Rahman K. Studies on free radicals, antioxidants, and co"‘factors. Clin Interv Aging 2007;2:219"‘36.

Dickinson DA, Forman HJ. Cellular glutathione and thiols metabolism. Biochem Pharmacol 2002;64:1019"‘26.

Sugunadevi G, Suresh K, Vijayaanand MA, Rajalingam K, Sathiyapriya J. Anti genotoxic effect of Mosinone"‘A on 7, 12"‘dimethyl benz[a] anthracene induced genotoxicity in male golden Syrian hamsters. Pathol Oncol Res 2012;18:69"‘77.

Cerutti PA. Prooxidant states and tumor promotion. Science 1985;227:375"‘81.

Percival M. Phytonutrients and detoxification. Clin Nutr Insights 1997;5:1"‘4.

Baer"‘Dubowska W, S z a e fer H. Modulat i o n o f carcinogen"‘metabolizing cytochromes P450 by phytochemicals in humans. Expert Opin Drug Metab Toxicol 2013;9:927"‘41.

Kumar R, Kaur R, Singh AP, Arora S. Diminution of hepatic response to 7, 12"‘dimethylbenz(α) anthracene by ethyl acetate fraction of Acacia catechu willd. through modulation of xenobiotic and anti"‘oxidative enzymes in rats. PLoS One 2014;9:e90083.