Oxidative Stress Induced by Lead and Antioxidant Potential of Certain Adaptogens in Poultry
Authors
-
Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad - 500030 ,IN
M. Ratan Kumar -
Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad - 500030 ,INA. Gopala Reddy
-
Department of Veterinary Pathology, College of Veterinary Science, Rajendranagar, Hyderabad - 500030 ,INY. Anjaneyulu
-
Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad - 500030 ,ING. Dilip Reddy
Keywords:
Amelioration by adaptogens, lead, oxidative stressAbstract
Effect of lead was studied for its action on antioxidant defense in broilers. A total of 225 one-day-old male broiler chicks (Vencobb strain) were divided randomly into 15 groups consisting of 15 chicks in each group. Group 1 was maintained on basal diet, group 2 on polyherbal formulation (PHF; stressroak), group 3 on shilajit, group 4 on amla, and group 5 on vitamin E + selenium (Se). Group 6 was maintained on lead for 42 days (6 weeks) and group 7 on lead for 28 days and subsequently on basal diet without lead for the remaining two weeks. Groups 8, 9, 10, and 11 were given lead along with PHF, shilajit, amla, and vitamin E + Se, respectively throughout the experiment for 6 weeks. Groups 12, 13, 14, and 15 were given lead containing diet for the first four weeks (28 days) and subsequently treated with PHF, shilajit, amla, and vitamin E + Se, respectively for the remaining two weeks. Antioxidant status of the birds was analyzed by assaying blood samples for glutathione (GSH) peroxidase, GSH reductase, and catalase at the end of fourth and sixth weeks, whereas Thiobarbituric acid reacting substances (TBARS) and GSH concentrations were estimated in liver homogenate at the end of the sixth week. The antioxidant defense parameters were significantly altered in toxic control groups indicating the possible oxidative damage caused by lead, whereas the parameters were normal in control groups 1 to 5 and other groups that were given the drugs in test, indicating their good ameliorating activity in oxidative stress.Downloads
Published
How to Cite
Issue
Section
Accepted 2018-05-15
Published 2018-05-16
References
Loguerico C, De Girolamo V, De Sio I, Tuccillo C, Ascione A, Baldi F, et al. Non alcoholic fattyliver disease in an area of southern Italy: Main clinical, histological and patho physiological aspects. J Hepatol 2001;35:568-574.
Farrukh A, Iqbal A, Zafar M. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turk J Biol 2006;30:177-83.
Kamashi K, Reddy AG, Reddy KS, Reddy VR. Evaluation of zinc against salinomycin toxicity in broilers. Indian J Physiol Pharmacol 2004;48:89-95.
Paglia DE, Valentine WN. Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967;70:158-69.
Raghuramulu N. Manual of Laboratory Techniques. Hyderabad: National Institute of Nutrition (ICMR); 1983. p. 204-6.
Britton C, Machlly AC. Assay of catalase and peroxidases. Methods Enzymol 1956;136:765-8.
Balasubramanian KA, Manohar M, Mathan VI. An unidentified inhibitor of lipid peroxidation in intestinal mucosa. Biochim Biophys Acta 1988;962:51-8.
Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione - s- transferase in rat lung and liver. Biochim Biophys Acta 1979;582:67-8.
Snedecor WG, Cochran GW. Statistical Methods. 8th ed. USA: Iowa State University Press; 1994.
Gurer H, Ercal N. Can antioxidants be beneficial in the treatment of lead poisoning? Free Radic Biol Med 2000;29:927-45.
Russo A, Izzo AA, Cardile V, Borrelli F, Vanella A. Indian medicinal plants as antiradicals and DNA cleavage protectors. Phytomedicine 2001;8:125-32.
Devi PU, Ganasoundari A. Modulation of glutathione and antioxidant enzymes by Ocimum sanctum and its role in protection against radiation injury. Indian J Exp Biol 1999;37:262-8.
Halim EM, Mukhopadhyay AK. Effect of Ocimum sanctum (tulsi) and vitamin E on biochemical parameters and retinopathy in streptozotocin induced diabetic rats. Indian J Clin Biochem 2006;21:181-8.
Bhattacharya A, Ghosal S, Bhattacharya SK. Antioxidant activity of tannoid principles of Emblica officinalis (amla) in chronic stress induced changes in rat brain. Indian J Exp Biol 2000;38:877-80.
Martinez G, Delgado R, Perez G, Garrido G, Nunez Selles AJ, Leon OS. Evaluation of the in vitro antioxidant activity of Mangifera indica L. extract (Vimang). Phytother Res 2000;14:424-7.
Bhattacharya SK, Bhattachrya A, Chakrabarti A. Adaptogenic activity of Siotone, a polyherbal formulation of Ayurvedic rasayanas. Indian J Exp Biol 2000;38:119-28.
Devasagayam TP, Kamat JP, Nair S, Sreejayam N, Nesaretnam K, Packer L. Antioxidant action of curcumin. Micronutrients and health: Molecular Biological Mechanisms. In: Nesaretnam K, Packer L, Editors. USA: AOCS, Press Champaign, IL; 2001. p. 42-59.
Gupta S, Mediratte PK, Singh S, Sharma KK, Shukla R. Antidiabetic, antihypercholesterolaemic and antioxidant effect of Ocimim sanctum (Linn) seed oil. Indian J Exp Biol 2006;44:300-4.
Kumaran A, Karunakaran RJ. Nitric oxide radical scavenging active component from Phyllanthus emblica L. Plant Foods Hum Nutr 2006;61:1-5.
Hernández P, Delgado R, Walczak H. Mangifera indica L. extract protects T cells from activation-induced cell death. Int Immunopharmacol 2006;6:1496-505.
Bansal AK, Bansal M, Soni G, Bhatnagar D. Protective role of Vitamin E pre-treatment on N-nitrosodiethylamine induced oxidative stress in rat liver. Chem Biol Interact 2005;156:101-11.
