Cold Stress Offered Modulation on Chlorpyrifos Toxicity in Aging Rat Central Nervous System
Authors
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Department of Zoology, Bangalore University, Bangalore, Karnataka ,IN
Mahaboob Basha -
Government College for Boys, Kolar, Karnataka ,INAnnappa Poojary
Keywords:
Acetylcholinesterase, ATPases, ChAT, chlorpyrifos, CNS, cold stress, interactive effectsAbstract
The adverse effects produced by chlorpyrifos (CPF) or cold stress alone in humans and animals are well documented, but there is no information available relating to the consequences of their co- exposure in an age-related manner. In this study, effects of sublethal doses of CPF were carried out in vivo, for 48 h to assess the biochemical perturbations in relation to interactions with cold stress (15°C and 20°C) in different age group rat CNS. A positive interaction of CPF with age of animal and cold exposure was observed resulting in marked decrease in the activity levels of AChE (P<0.05), ChAT (P<0.05), Na+, K+-ATPase (P<0.05), Ca2+-ATPase (P<0.05), and Mg2+-ATPase (P<0.05). The ANOVA and posthoc analysis showed that regulatory enzymes decreased significantly (P<0.05) on CPF exposure. Overall, the effect of co-exposure was appreciably different from either of the exposures. Synergistic interaction of CPF and cold stress at 15°C showed higher inhibition in comparison with CPF and cold stress alone and together at 20°C. Further, this study reveals that young animals are significantly vulnerable and sensitive than adults.Downloads
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Accepted 2018-05-24
Published 2018-05-25
References
Richardson RJ, Moore TB, Kayyali US, Randall JC. Chlorpyrifos: Assessment of potential for delayed neurotoxicity by repeated dosing in adult hens with monitoring of brain acetylcholinesterase, brain and lymphocyte neurotoxic esterase and plasma butyrylcholinesterase activities. Fundam Appl Toxicol 1993;21:89-96.
Mehta A, Verma RS, Srivastava N. Chlorpyrifos – induced alterations in rat brain acetylcholinesterase, lipid peroxidation and ATPases. Indian J Biochem Biophys 2005;42:54-8.
Namba T, Nolte CT, Jackrel J, Grob D. Poisoning due to organophosphate insecticides: Acute and chronic manifestations. Am J Med 1971;50:475-92.
Slotkin TA, Cousins MM, Tate CA, Seidler FJ Persistent cholinergic presynaptic deficits after neonatal chlorpyrifos exposure. Brain Res 2001;902:229-43.
Alvarez AA, Ramirez-San JE, Canizales-Roman A. Chlorpyrifos induces oxidative stress in rats. Toxicol Environ Chem 2008;90:1019-25.
Mansour SA, Mossa AH. Oxidative damage, biochemical and histopathological alterations in rats exposed to chlorpyrifos and the antioxidant role of zinc. Pest Biochem Physiol 2010;96:14-23.
Dhanalakshmi S, Srikumar R, Manikandan S, Parthasarathy NJ, Devi RS. Antioxidant Property of Triphala on Cold Stress Induced Oxidative Stress in Experimental Rats. J Health Sci 2006;52:843-7.
Maguire CC, Williams BA. Response of thermal stressed bobwhite to organophosphorus exposure. Environ Pollut 1996;11:101-17.
Wahba ZZ, Soliman K F A. Effect of stress on choline acetyltransferase activity of the brain and the adrenal of the rat. Experientia 1992;48:265-8.
Basha PM, Poojary A. Chlorpyrifos induced region specific vulnerability in rat CNS and modulation by age and cold stress: An interactive study. Neurochem Res 2011;36:241-9.
Rauh VA, Garfinkel R, Perera FP, Andrews HF, Hoepner L, Barr DB, et al. Impact of prenatal chlorpyrifos exposure on neurodevelopment in the first 3 years of life among inner-city children. Pediatrics 2006;118: E1845-59.
Boon PE, Van der Voet H, Van Raaij MT, Van Klaveren JD. Cumulative risk assessment of the exposure to organophosphorus and carbamate insecticides in the Dutch diet. Food Chem Toxicol 2008;46:3090-8.
Zheng Q, Olivier K, Won YK, Pope CN. Comparative cholinergic neurotoxicity of oral chlorpyrifos exposures in pre-weanling and adult rats. Toxicol Sci 2000;55:124-32.
Moser VC, Chanda SM, Mortensen SR, Padilla S. Age- and gender-related differences in sensitivity to chlorpyrifos in the rat reflect developmental profiles of esterase activities. Toxicol Sci 1998;46:211-22.
Finney DJ. Probit analysis. 3rd ed. London: Cambridge University Press; 1971. p. 333.
Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM. A new and rapid calorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961;7:88-95.
Morris D. The effect of sulphydril and other disulphide reducing agents on choline acetyltransferase activity estimated with synthetic acetyl-CoA. J Neurochem 1967;14:19-27.
Yamaguchi I, Matsumura F, Kadous AA. Inhibition of synaptic ATPases by heptachlorepoxide in rat brain. Int J Pestic Biochem Physiol 1979;11:285-93.
Fiske CH, Subbarao Y. The colorimetric determination of phosphorous. J Biol Chem 1925;66:375-400.
Lowry OH, Rosebrough NJ, Farr AL, Randal R. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75.
Rusyniak DE, Nanagas KA. Organophosphate poisoning. Semin Neurol 2004;24:197-204.
Suresh A, Sivaramakrishna B, Victoriamma PC, Radhakrishnaiah K. Comparative study on the inhibition of acetylcholinesterase activity in the fresh water fish Cyprinus carpio by mercury and zinc. Biochem Int 1992;29:367-75.
Schuurmans SF, Bontig SL. Transport adenosine triphosphotases: Properties and functions. Physiol Rev 1981;61:1-76.
Lehninger AL, Biochemistry. 2nd ed. Ludhiana: Kalyani Publication; 1980.
Owens MJ Nemeroff CV. Philosophy and Pharmacology of corticotrophin releasing factor. Pharmacol Rev 1991; 91:425-73.
McInntosh LJ, Sapolsky RM. Glucocorticoids increased the accumulation of reactive oxygen species and enhance adriamycin-induced toxicity in neuronal culture. Exp Neurol 1996;141:201-6.
Benke GM, Murphy SD. The influence of age on the toxicity and metabolism of methyl parathion and parathion in male and female rats. Toxicol Appl Pharmacol 1975;31:254-69.
Rattner BA, Franson JC. Methyl parathion and fenvalerate toxicity in American Kestrels: Acute physiological responses and effects of cold. Can J Physiol Pharmacol 1984;62: 787-92.
