In-Vivo Study of Protective Role of Moringa oleifera Aqueous Pod Extract in Cisplatin Induced Nephrotoxicity

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Authors

  • Rupal Purena
     Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, Bilaspur – 495009, Chhattisgarh ,IN
  • Renu Bhatt
     Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, Bilaspur – 495009, Chhattisgarh ,IN

DOI:

https://doi.org/10.18311/ti/2018/21675

Keywords:

Cisplatin, M. oleifera, Moringaceae, Nephrotoxicity, Oxidative Stress, Pod Extract.

Abstract

The present study investigates aqueous pod extract of Moringa oleifera for its protective role in cisplatin induced nephrotoxicity. The experiment was designed for 14 days and male wistar rats were used as experimental animal. Rats were divided into 7 groups (n = 6). Group 1 (normal control), group 2 (vehicle control), group 3 (cisplatin control) given cisplatin, 12 mg/kg Body Weight (BW) intraperitoneally in 0.9% saline on 11th day of treatment. Group 4 to 7 was given pod extracts via oral gavage in different doses 200 mg/kg and 400 mg/kg BW form day 1-13, group 6 and 7 was also given cisplatin (12 mg/kg BW) on day 11. On 14th day blood sample were collected for serum analysis and all animals were sacrificed to study biochemical markers of renal dysfunction and histopathology study. Serum analysis of creatinine and blood urea nitrogen showed treatment with lower dose (i.e., 200mg/kg BW) significantly lowers the enhanced levels in cisplatin treated rats. Biochemical analysis showed lower dose significantly enhanced renal Catalase, SOD, and GPx activity while higher dose significantly enhances GPx and lowers renal MDA level in treated rats. The extract also improves histological damage. The study concluded that M. oleifera aqueous pod extract reduces renal damage caused by cisplatin treatment.

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Published

2019-05-15

How to Cite

Purena, R., & Bhatt, R. (2019). <i>In-Vivo</i> Study of Protective Role of <i>Moringa oleifera</i> Aqueous Pod Extract in Cisplatin Induced Nephrotoxicity. Toxicology International, 25(1), 19–25. https://doi.org/10.18311/ti/2018/21675

Issue

Volume 25, Issue 1, January-March 2018

Section

Research Articles
Crossref
Scopus
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Europe PMC
Received 2018-08-03
Accepted 2018-09-20
Published 2019-05-15

 

References

Pabla N, Dong Z. Cisplatin nephrotoxicity: Mechanisms and renoprotective strategies. Kidney Int. 2008; 73(9):994– 1007. https://doi.org/10.1038/sj.ki.5002786 PMid:18272962

Rosenberg B. Charles F. Kettring prize. Fundamental studies with cisplatin. Cancer. 1985; 55(10):2303–16. https://doi.org/10.1002/1097-0142(19850515)55:10<2303::AIDCNCR2820551002> 3.0.CO;2-L

Ries F, Klastersky J. Nephrotoxicity induced by cancer chemotherapy with special emphasis on cisplatin toxicity. Am J Kidney Dis. 1986; 8(5):368–79. https://doi.org/10.1016/ S0272-6386(86)80112-3

Arjumand W, Seth A, Sultana S. Rutin attenuates cisplatin induced renal inflammation and apoptosis by reducing NFκB, TNF-α and caspase-3 expression in wistar rats. Food Chem Toxicol. 2011; 49(9):2013–21. https://doi.org/10.1016/j.fct.2011.05.012 PMid:21605616

Chang B, Nishikawa M, Sato E, Utsumi K, Inoue M. L-Carnitine inhibits cisplatin-induced injury of the kidney and small intestine. Arch Biochem Biophys. 2002; 405(1):55– 64. https://doi.org/10.1016/S0003-9861(02)00342-9

Purena R, Seth R, Bhatt R. Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats. Toxicol Rep. 2018; 5: 270–7. https:// doi.org/10.1016/j.toxrep.2018.01.008 PMid:29487802 PMCid:PMC5814364

Gomez-Sierra T, Eugenio-Perez D, Sanchez-Chinchillas A, Pedraza-Chaverri J. Role of food-derived antioxidants against cisplatin induced-nephrotoxicity. Food Chem Toxicol. 2018; 120:230–42. https://doi.org/10.1016/j.fct.2018.07.018 PMid:29990577

Antunes LMG, Darin JDAC, Bianchi MDLP. Protective effects of vitamin C against cisplatin-induced nephrotoxicity and lipid peroxidation in adult rats: A dose-dependent study. Pharmacol Res. 2000; 41(4):405–11. https://doi.org/10.1006/phrs.1999.0600 PMid:10704263

Davis CA, Nick HS, Agarwal A. Manganese superoxide dismutase attenuates cisplatin-induced renal injury: importance of superoxide. J Am Soc Nephrol. 2001; 12(12):2683–90. PMid:11729237

Lin M, Zhang J, Chen X. Bioactive flavonoids in Moringa oleifera and their health-promoting properties. J Funct Foods. 2018; 47:469–79. https://doi.org/10.1016/j.jff.2018.06.011

Anwar F, Latif S, Ashraf M, Gilani, AH. Moringa oleifera: A food plant with multiple medicinal uses. Phytother Res. 2007; 21(1):17–25. https://doi.org/10.1002/ptr.2023 PMid:17089328

Babu SC. Rural nutrition interventions with indigenous plant foods-a case study of vitamin A deficiency in Malawi. Biotechnol Agron Soc. 2000; 4(3):169–79.

Gopalakrishnan L, Doriya K, Kumar DS. Moringa oleifera: A review on nutritive importance and its medicinal application. Food Sci Human Wellness. 2016; 5(2):49–56. https:// doi.org/10.1016/j.fshw.2016.04.001

Abdulkadir AR, Zawawi DD, Jahan MS. DPPH antioxidant activity, total phenolic and total flavonoid content of different part of Drumstic tree (Moringa oleifera Lam). J Chem Pharm Res. 2015; 7(4):1423–8.

Paliwal R, Sharma V, Sharma SH. Hepatoprotective and antioxidant potential of Moringa oleifera pods against DMBA-induced hepatocarcinogenesis in male mice. Int J Drug Develop Res. 2011a; 3(2):128–38.

Paliwal R, Sharma V, Pracheta SS, Yadav S, Sharma SH. Anti-nephrotoxic effect of administration of Moringa oleifera Lam in amelioration of DMBA-induced renal carcinogenesis in Swiss albino mice. Bio Med. 2011b; 3(2):27–35.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265–75. PMid:14907713

Masayasu M, Hiroshi Y. A simplified assay method of superoxide dismutase activity for clinical use. Clin Chim Acta. 1979; 92(3):337–42. https://doi.org/10.1016/00098981(79)90211-0

Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70(1):158–69. PMid:6066618

Cohen G, Dembiec D, Marcus J. Measurement of catalase activity in tissue extracts. Anal Biochem. 1970; 34(1):30–8. https://doi.org/10.1016/0003-2697(70)90083-7

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2):351–8. https://doi.org/10.1016/00032697(79)90738-3

Atessahin A, Yilmaz S, Karahan I, Ceribasi AO, Karaoglu A. Effects of lycopene against cisplatin-induced nephrotoxicity and oxidative stress in rats. Toxicol. 2005; 212(2):116–23. https://doi.org/10.1016/j.tox.2005.04.016 PMid:15946783

Atessahin A, Ceribasi AO, Yuce A, Bulmus O, Cikim G. Role of ellagic acid against cisplatin"induced nephrotoxicity and oxidative stress in rats. Basic Clin Pharmacol Toxicol. 2007; 100(2):121–6. PMid:17244261

Karimi G, Ramezani M, Tahoonian Z. Cisplatin nephrotoxicity and protection by milk thistle extract in rats. Evid Based Complement Alternat Med. 2005; 2(3):383–6. https://doi.org/10.1093/ecam/neh103 PMid:16136217 PMCid:PMC1193544

Chirino YI, Hernandez-Pando R, Pedraza-Chaverri J. Peroxynitrite decomposition catalyst ameliorates renal damage and protein nitration in cisplatin-induced nephrotoxicity in rats. BMC Pharmacol 2004; 4(20):1–9.

Yilmaz HR, Iraz M, Sogut S, Ozyurt H, Yildirim Z, Akyol O, Gergerlioglu S. The effects of erdosteine on the activities of some metabolic enzymes during cisplatin-induced nephrotoxicity in rats. Pharmacol Res. 2004; 50(3):287–90. https://doi.org/10.1016/j.phrs.2004.03.003 PMid:15225672

Sahu BD, Rentam KKR, Putcha UK, Kuncha M, Vegi GMN, Sistla R. Carnosic acid attenuates renal injury in an experimental model of rat cisplatin-induced nephrotoxicity. Food Chem Toxicol. 2011; 49(12):3090–7. https://doi.org/10.1016/j.fct.2011.08.018 PMid:21930180

Lee HS, Kim BK, Nam Y, Sohn UD, Park ES, Hong SA, Lee YH, Jeong JH. Protective role of phosphatidylcholine against cisplatin-induced renal toxicity and oxidative stress in rats. Food Chem Toxicol. 2013; 58:388–93. https://doi.org/10.1016/j.fct.2013.05.005 PMid:23684996

Ashok Kumar N, Pari L. Antioxidant action of Moringa oleifera Lam.(drumstick) against antitubercular drugs induced lipid peroxidation in rats. J Med Food. 2003; 6(3):255–9. https://doi.org/10.1089/10966200360716670 PMid:14585192

Bharali R, Tabassum J, Azad MRH. Chemomodulatory effect of Moringa oleifera, Lam, on hepatic carcinogen metabolising enzymes, antioxidant parameters and skin papillomagenesis in mice. Asian Pac J Cancer Preven. 2003; 4(2):131–9. PMid:12875626