Allergic Effects of Airborne Fungal Conidia on BALB/c Mice

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Authors

  • Madhu Manikpuri
     ,IN
  • Rajesh Anand
     ,IN
  • Bhupendra N. Tiwary
     ,IN

DOI:

https://doi.org/10.18311/ti/2018/v25i3/23525

Keywords:

Aeroallergens, Allergens, Allergic Rhinitis

Abstract

Airborne fungal spores are one of the major causes of human respiratory disorders that are responsible for allergic reactions, asthma and respiratory tract infections. Determination of total and specific serum Immunoglobulin E (IgE) level has been widely applied to the diagnosis of allergic diseases including allergy caused by fungi. The present study was carried out to assess the allergenic potential of dominant airborne fungal spores and its impact on the respiratory system. The BALB/c mice were sensitized intranasally with the conidial suspension of six selected fungal stains and the allergic potential was evaluated on the basis of serum IgE, differential blood cell counts and histopathology of the lung tissues from mice receiving post sensitization doses. A significant increase in the total serum IgE level was observed in the serum of all mice as compared to the control. Amongst the fungi used in the study, Aspergillus terreus showed the maximum increase in the level of serum IgE of treated animals. The differential count revealed the highest increase in the eosinophil count in mice treated with Aspergillus terreus and Penicillium sp. The histopathological analysis of the lungs of treated animals showed increased infiltration of blood cells and thickening of airway epithelia. The observation of the study indicated that spores of Aspergillus terreus have greater allergic potential than all other airborne fungi.

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Published

2019-10-22

How to Cite

Manikpuri, M., Anand, R., & Tiwary, B. N. (2019). Allergic Effects of Airborne Fungal Conidia on BALB/c Mice. Toxicology International, 25(3), 155–162. https://doi.org/10.18311/ti/2018/v25i3/23525

Issue

Volume 25, Issue 3, July -September 2018

Section

Articles
Crossref
Scopus
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Europe PMC
Received 2019-04-06
Accepted 2019-05-27
Published 2019-10-22

 

References

Chang ML, Cui C, Liu YH, Pei LC, Shao B. Analysis of total immunoglobulin E and specific immunoglobulin E of 3,721 patients with allergic disease. Biomed Rep. 2015; 4:5737. https://doi.org/10.3892/br.2015.455. PMid:26171168.PMCid:PMC4486958.

Buskirk AD, Green BJ, Lemons AR, Nayak AP, Goldsmith WT, Kashon ML, et al. Anderson SE, Hettick JM, Templeton SP, Germolec DR, Beezhold DH. A murine inhalation model to characterize pulmonary exposure to dry Aspergillus fumigatus conidia. PloS one 2014; 9:1-13. https://doi.org/10.1371/journal.pone.0109855. PMid:25340353..PMCid:PMC4207673.

Pandey S, Hoselton SA, Schuh JM. The impact of Aspergillus fumigatus viability and sensitization to its allergens on the murine allergic asthma phenotype. Bio Med Res Int.2013; 2013:1-17. https://doi.org/10.1155/2013/619614.PMid:24063011. PMCid:PMC3770015.

Galli SJ, Tsai M. IgE and mast cells in allergic disease. Nat Med. 2012; 18:693. https://doi.org/10.1038/nm.2755.PMid:22561833. PMCid:PMC3597223.

Horner WE, Helbling A, Salvaggio JE. Fungal allergens. Clin Microbiol Rev. 1995; 8:161-79. https://doi.org/10.1128/ CMR.8.2.161. PMid:7621398. PMCid:PMC172854.

Havaux X, Zeine A, Dits A, Denis O. A new mouse model of lung allergy induced by the spores of Alternaria alternata and Cladosporium herbarum molds. J Clin Exp Immuno. 2005; 139:179-88. https://doi.org/10.1111/j.13652249.2004.02679.x. PMid:15654816. PMCid:PMC1809297.

Porter PC, Roberts L, Fields A, Knight M, Qian Y, Delclos GL, et al. Necessary and sufficient role for T helper cells to prevent fungal dissemination in allergic lung disease.Infect Immun. 2011; 79:4459-71. https://doi.org/10.1128/ IAI.05209-11. PMid:21875960. PMCid:PMC3257943.

Asif AR, Oellerich M, Amstrong VW, Riemenschneider B, Monod M, Reichard U. Proteome of conidial surface associated proteins of Aspergillus fumigatus reflecting potential vaccine candidates and allergens. J Proteome Res. 2006; 5:954-62. https://doi.org/10.1021/pr0504586.PMid:16602703 .

Abu-Dieyeh MH, Barham R, Abu-Elteen K, Al-Rashidi R, Shaheen I. Seasonal variation of fungal spore populations in the atmosphere of Zarqa area, Jordan. Aerobiologia. 2010; 26:263-76. https://doi.org/10.1007/s10453-010-9162-2.

Sinclair D, Peters SA. The predictive value of total serum IgE for a positive allergen specific IgE result. J Clin Pathol.2004; 57:956-9. https://doi.org/10.1136/jcp.2004.017681.PMid:15333657. PMCid:PMC1770430.

Hachimura S, Ametani A, Sato T, Kumagai Y, Takamizawa K, Habu S, et al. Serum IgE response to orally ingested antigen: a novel IgE response model with allergen-specific T-cell receptor transgenic mice. J Allergy Clin Immuno. 2000; 105:788-95. https://doi.org/10.1067/mai.2000.104934.PMid:10756231 .

Ito R, Gon Y, Nunomura S, Atsuta R, Harada N, Hattori T, et al. Development of assay for determining free IgE levels in serum from patients treated with omalizumab.Allergol Int. 2014; 63:37-47. https://doi.org/10.2332/ allergolint.13-OA-0643. PMid:24809374 .

Ferreira MA. Cytokine expression in allergic inflammation: Systematic review of in vivo challenge studies. Mediators Inflamm. 2003; 12:259-67. https://doi.org/10.1080/096293 50310001619717. PMid:14760932. PMCid:PMC1781628.

Hamilton RG, Adkinson Jr NF. Clinical laboratory assessment of IgE-dependent hypersensitivity. J Allergy Clin Immunol. 2003; 111:687-701. https://doi.org/10.1067/ mai.2003.123. PMid:12592314 .

Saldanha JC, Gargiulo DL, Silva SS, Carmo-Pinto FH, Andrade MC, Alvarez-Leite JI, et al. A model of chronic IgE-mediated food allergy in ovalbumin-sensitized mice. Braz J Med Biol Res. 2004; 37:809-16. https://doi.org/10.1590/S0100-879X2004000600005. PMid:15264023 .

Hamelmann E, Vella AT, Oshiba A, Kappler JW, Marrack P, Gelfand EW. Allergic airway sensitization induces T cell activation but not airway hyperresponsiveness in B cell- deficient mice (IgEymAb). Proc Natl Acad Sci.1997; 94:1350-5. https://doi.org/10.1073/pnas.94.4.1350.PMid:9037056. PMCid:PMC19794.

Manikpuri M, Tiwari KL, Tiwary BN. Effect of seasonal variation on aeromycoflora of Bilaspur, Chhattisgarh, involved in allergic reactions. Aerobiologia. 2018; 34:11926. https://doi.org/10.1007/s10453-017-9500-8.

Anand R, Tiwary BN. Cytokine profile and cytotoxicity in response to acute intratracheal dose of Metarhizium anisopliae in BALB/c mice. Med. Mycol 2010; 48:103948. https://doi.org/10.3109/13693786.2010.494254.PMid:20586678 .

Zetterstrom O, Nordvall SL, Bjorkstéen B, Ahlstedt S, Stelander M. Increased IgE antibody responses in rats exposed to tobacco smoke. J Allergy Clin Immunol. 1985; 75:594-98. https://doi.org/10.1016/0091-6749(85)90035-1.

Van der Spuy WJ, Potgieter M, Pretorius E, Vieira WA. Differential leukocyte counts of SJL/J mice with dysferlinopathy treated with resveratrol and coenzyme Q10. Scand J Am Assoc Lab Anim Sci. 2010; 37:1-7..

Raj J, Chandra M, Dogra TD, Pahuja M, Raina A.Determination of median lethal dose of combination of endosulfan and cypermethrin in wistar rat. Toxicol Int.2013; 20:1-5. https://doi.org/10.4103/0971-6580.111531.PMid:23833430. PMCid:PMC3702116.