Allopurinol Reduced Oleic Acid Plasma on High-fat Diet-induced Hypertensive Rats

Abstract

Hypertension is still a big challenge and is a key risk factor for cardiovascular disease which is the leading cause of death in the world. We evaluate the effects of Allopurinol in high-fat diet-induced hypertensiverats. To this end we induced hypertension of SD rats by high fat-diet for six weeks and divided into 6 groups (n = 5), for six weeks and one group was fed standard chow. Drugs were given for seven days orally; one group was only given a high-fat diet and CMC 0.5%, as Negative Control; one group was given a high-fat diet and Isosorbide Dinitrate (ISDN) 18 mg/kg as Positive Control, three groups were given a high-fat diet and Allopurinol with dose range of 45-180 mg/kg. Systolic, diastolic, mean arterial pressure and blood glucose were compared between diet groups and normal group; Free Fatty Acid Plasma and Amino Acid Plasma were determined by GC-MS. As a results, Allopurinol significantly reduced blood pressure in all three dose groups (p<0.05). The dose group of Allopurinol and ISDN gave similar results in lowering blood pressure and glucose levels which measured on the last day of drug administration. Allupurinol also reduced oleic acid plasma compared with high-fat diet group. In conclusion, Allopurinol has a potential as an antihypertensive agent by modulating linoleic acid.