Sub-Acute Toxicity Study of Hydro-Alcoholic Extracts of Sansevieria cylindrica and Plumeria obtusa in Wistar Albino Rats

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Authors

  • Sunil Shewale
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Savitribai Phule University of Pune, Pune – 411018, Maharashtra ,IN
  • Vaishali Undale
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Savitribai Phule University of Pune, Pune – 411018, Maharashtra ,IN
  • V. Addepalli
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Savitribai Phule University of Pune, Pune – 411018, Maharashtra ,IN
  • Vrushali Bhalchim
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Savitribai Phule University of Pune, Pune – 411018, Maharashtra ,IN
  • Shivani Desai
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Savitribai Phule University of Pune, Pune – 411018, Maharashtra ,IN
  • Vikas Wawale
     Department of Quality Assurance, QMS, Synapse Labs. Ltd., Pune – 411014, Maharashtra ,IN
  • Maruti Shelar
     Department of Pharmacognosy, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Savitribai Phule University of Pune, Pune – 411018, Maharashtra ,IN
  • Shubham Padole
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Savitribai Phule University of Pune, Pune – 411018, Maharashtra ,IN
  • Shital Satone
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Savitribai Phule University of Pune, Pune – 411018, Maharashtra ,IN
  • Shweta Lembhe
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Savitribai Phule University of Pune, Pune – 411018, Maharashtra ,IN
  • Sameer Parekh
     Department of Clinical Research and Pharmacovigilance, Serum Institute of India Pvt. Ltd., Pune – 411028, Maharashtra ,IN
  • Pramod Pujari
     Department of Clinical Research and Pharmacovigilance, Serum Institute of India Pvt. Ltd., Pune – 411028, Maharashtra ,IN

DOI:

https://doi.org/10.18311/ti/2023/v30i4/31062

Keywords:

Plumeria obtusa, Safety, Sansevieria cylindrica, Sub-Acute Toxicity, Toxicity
Pharmacology

Abstract

Sansevieria cylindrica Bojer ex. Hook (Asparagaceae) and Plumeria obtusa L. (Apocynaceae) are useful traditional medicines. The present study assessed the toxicity of a combination of hydro-alcoholic extract of aerial parts of both these plants through repeat administration in Wistar albino rats. This study is an extension of an acute toxicity study, wherein female rats were orally administered with a single dose of 2000mg/kg of both plant extracts. Sub-acute toxicity of both plants (200 and 400 mg/kg p.o.) was studied by daily dosing of Wistar rats of both sexes for 28 days. Toxicity, mortality, body weight changes, food and water intake, and neurological assessments were recorded. Hematological, biochemical, and urine analysis was done before and after dosing on day 29. Absolute and relative organ weight and histological evaluation were performed on day 29. The acute toxicity study revealed no lethal effects indicating that LD50 is greater than 2000mg/ kg. The sub-acute study observed a significant increase (p < 0.05) in body weight, feed, and water intake. The body weight gain in rats from the treatment group was non-significant (p > 0.05) compared to the control group. A significant (p < 0.05) increase in lymphocytes, granulocytes, RBC, platelet, creatinine, albumin, triglycerides, and alkaline phosphatase were also observed. The evaluation of different neurobehavior parameters showed a significant increase (p < 0.05). Histopathological analysis showed mild liver distortion in male rats in both treatment groups. Most of the significant observations were considered incidental findings indicative of low toxicity of plant extract during long-term use.

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Published

2023-10-31

How to Cite

Shewale, S., Undale, V., Addepalli, V., Bhalchim, V., Desai, S., Wawale, V., Shelar, M., Padole, S., Satone, S., Lembhe, S., Parekh, S., & Pujari, P. (2023). Sub-Acute Toxicity Study of Hydro-Alcoholic Extracts of <i>Sansevieria cylindrica</i> and <i>Plumeria obtusa</i> in Wistar Albino Rats. Toxicology International, 30(4), 537–558. https://doi.org/10.18311/ti/2023/v30i4/31062

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Volume 30, Issue 4, October-December 2023

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Copyright (c) 2023 Toxicology International

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Received 2022-08-25
Accepted 2023-10-08
Published 2023-10-31

 

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