Testicular Toxicity following Subacute Exposure of Arsenic and Mancozeb alone and in Combination: Ameliorative Efficacy of Quercetin and Catechin

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Authors

  • Rasia Yousuf
     Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Science and Animal Husbandry, R. S. Pura - 181102, Jammu and Kashmir ,IN
  • Pawan Kumar Verma
     Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Science and Animal Husbandry, R. S. Pura - 181102, Jammu and Kashmir ,IN
  • Priyanka Sharma
     Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Science and Animal Husbandry, R. S. Pura - 181102, Jammu and Kashmir ,IN
  • Shilpa Sood
     Division of Veterinary Pathology, Faculty of Veterinary Science and Animal Husbandry, R. S. Pura - 181102, Jammu and Kashmir ,IN
  • N. K. Pankaj
     Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Science and Animal Husbandry, R. S. Pura - 181102, Jammu and Kashmir ,IN
  • Sanjay Agarwal
     Division of Animal Reproduction and Gynaecology, Faculty of Veterinary Science and Animal Husbandry, R. S. Pura - 181102, Jammu and Kashmir ,IN

DOI:

https://doi.org/10.18311/ti/2023/v30i3/32276

Keywords:

Arsenic, Catechin, Mancozeb, Quercetin, Testicular Damage

Abstract

Mancozeb (MZ) is a contact fungicide having low toxicity in non-target species, but its continuous exposure can be harmful. The aim of the present study was to determine the impact of the toxic interaction between MZ and arsenic on the testicular tissue of rats and to compare the amelioration potential of quercetin and catechin against the induced toxicity. Sixty adult rats were randomly allocated into 10 groups with 6 animals in each. A significant (p<0.05) decline in TAS, TTH, SOD, CAT, GPx, GR and TTH and a rise (p<0.05) in MDA and AOPP-were recorded in testicular tissue of MZ-treated rats in comparison to control. Exposure to different doses of arsenic (10, 50, 100 ppb) also produced a dose-dependent effect on these oxidative biomarkers. Arsenic exposure produces potentiating MZ-induced testicular toxicity in Wistar rats. Testicular damage was further corroborated by extremely severe histopathological changes viz., interstitial as well as sub-capsular congestion, oedema aside from degeneration, necrosis and loss of seminiferous tubules and a drastic deterioration in sperm motility in this group. In contrast, administration of toxicants along with quercetin or catechin markedly attenuated the alterations in oxidative as well as cellular damage biomarkers and testicular histopathological alterations. Our results suggested that simultaneous low dose exposure to arsenic potentiated testicular toxicity induced by MZ. Furthermore, catechin was more potent as compared to quercetin in ameliorating testicular changes induced by concurrent arsenic and MZ exposure.

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Published

2023-09-20

How to Cite

Yousuf, R., Verma, P. K., Sharma, P., Sood, S., Pankaj, N. K., & Agarwal, S. (2023). Testicular Toxicity following Subacute Exposure of Arsenic and Mancozeb alone and in Combination: Ameliorative Efficacy of Quercetin and Catechin. Toxicology International, 30(3), 255–267. https://doi.org/10.18311/ti/2023/v30i3/32276

Issue

Volume 30, Issue 3, July-September 2023

Section

Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2023-01-01
Accepted 2023-06-30
Published 2023-09-20

 

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