Molybdenum-Induced Oxidative Stress and Histopathological Alterations in the Epididymis

Jump To References Section

Authors

  • Geeta Pandey
     Department of Zoology, IIS deemed to be University, Jaipur - 302020, Rajasthan ,IN
  • G. C. Jain
     Department of Zoology, University of Rajasthan, Jaipur - 302004, Rajasthan ,IN

DOI:

https://doi.org/10.18311/ti/2024/v31i2/34727

Keywords:

Antioxidants, Epididymis, Histology, Molybdenum, Rats

Abstract

Molybednum is one of the trace elements required for proper functioning of the human body. The present study was designed to assess the effects of molybdenum on accessory reproductive organ i.e. epididymis. Male Wistar rats were administered with three different doses of molybdenum (50, 100 and 150 mg/kg body weight) for 60 days. A recovery study was also conducted in the highest dose group for which half the animals of the group were left untreated for the next 60 days. Exposure to molybdenum induced a significant decline in superoxide dismutase, ascorbic acid and glutathione while lipid peroxidation revealed a significant increase in epididymis in dose-dependent manner. Significant reduction in epididymal epithelial cells height, short sterocilia, increased intertubular stroma, decreased epididymal sperm reserve in the lumen and reduction in the diameter of cauda epididymal tubules with a relatively large amount of intertubular stroma was observed in epididymis of mice treated with molybdenum which might be correlated with enhanced oxidative stress. In the recovery group, significant improvement in antioxidant parameters and histoarchitecture of epididymis was observed indicating toxic effects of molybdenum can be reversed by cessation of exposure.

Downloads

Download data is not yet available.

Downloads

Published

2024-05-09

How to Cite

Pandey, G., & Jain, G. C. (2024). Molybdenum-Induced Oxidative Stress and Histopathological Alterations in the Epididymis. Toxicology International, 31(2), 265–273. https://doi.org/10.18311/ti/2024/v31i2/34727

Issue

Volume 31, Issue 2, April-June 2024

Section

Articles

License

Copyright (c) 2024 Geeta Pandey, G. C. Jain

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Crossref
Scopus
Google Scholar
Europe PMC
Received 2023-08-14
Accepted 2024-03-16
Published 2024-05-09

 

References

Senger PL. Pathways to Pregnancy and Parturition. 2nd ed. Current Conceptions Inc; 2003.

Woodruff TJ, Jansen S, Giullette LJ, Guidice LC. Environmental impacts on reproductive health and fertility, New York: Cambridge University Press; 2010.

Sengupta P. Environmental and occupational exposure of metals and their role in male reproductive functions. Drug Chem Toxicol. 2013; 36(3):353-68. https://doi.org/10.3109/ 01480545.2012.710631 DOI: https://doi.org/10.3109/01480545.2012.710631

Balali-Mood M, Naseri K, Tahergorabi Z, Khazdair MR, Sadeghi M. Toxic mechanisms of five heavy metals: mercury, lead, chromium, cadmium, and arsenic. Front Pharmacol. 2021; 12:643972. https://doi.org/10.3389/ fphar.2021.643972 DOI: https://doi.org/10.3389/fphar.2021.643972

Nielsen FH. Trace and ultratrace elements, reference module in biomedical sciences. Elsevier; 2014. https://doi. org/10.1016/B978-0-12-801238-3.00235-X DOI: https://doi.org/10.1016/B978-0-12-801238-3.00235-X

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxide formation in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95:351-8. https://doi. org/10.1016/0003-2697(79)90738-3 DOI: https://doi.org/10.1016/0003-2697(79)90738-3

Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47:469−74. https://doi.org/10.1111/j.1432-1033.1974. tb03714.x DOI: https://doi.org/10.1111/j.1432-1033.1974.tb03714.x

Moron MJ, Depieare JW, Mannivik B. Levels of glutathione reductase and glutathione transferase activities in rat lung and liver. Biochem Biophys Acta. 1979; 582:67-8. https:// doi.org/10.1016/0304-4165(79)90289-7 DOI: https://doi.org/10.1016/0304-4165(79)90289-7

Roe JH, Kuether CA. Determination of ascorbic acid in whole blood and urine through the 2,4-DNPH derivative of dehydroascorbic acid. J Biol Chem. 1943; 147:399-407. https://doi.org/10.1016/S0021-9258(18)72395-8 DOI: https://doi.org/10.1016/S0021-9258(18)72395-8

Nasreldin M I, Leona GY, Otto F. Epididymal specificity and androgen regulation of rat EP2. Biology of Reprod. 2001; 65(2):575-80. https://doi.org/10.1095/biolreprod65.2.575 DOI: https://doi.org/10.1095/biolreprod65.2.575

Cornwell GA. New insights into epididymal biology and function. Hum Reprod Update. 2009; 15 (2):213-27. https:// doi.org/10.1093/humupd/dmn055 DOI: https://doi.org/10.1093/humupd/dmn055

Pandey R, Singh S. Effects of molybdenum on fertility of male rats. Biometals. 2002; 15:65–72. https://doi. org/10.1023/A:1013193013142 DOI: https://doi.org/10.1023/A:1013193013142

Lyubimov AV, Smith JA, Rousselleb SD, Merciecab MD, Tomaszewskic JE, Smith AC, Levinea BS. The effects of tetrathiomolybdate (TTM, NSC-714598) and copper supplementation on fertility and early embryonic development in rats. Reprod Toxicol. 2004; 19:223–33. https://doi.org/10.1016/j.reprotox.2004.07.006 DOI: https://doi.org/10.1016/j.reprotox.2004.07.006

Hamzeh M, Robaire B. Effect of testosterone on epithelial cell proliferation in the regressed rat epididymis. J Androl. 2009; 30(2):200-12. https://doi.org/10.2164/ jandrol.108.006171 DOI: https://doi.org/10.2164/jandrol.108.006171

Pandey G, Jain GC. Assessment of molybdenum induced alteration in oxidative indices, biochemical parameters and sperm quality in testis of Wistar male rats. Asian J Biochem. 2015; 10:267-80. https://doi.org/10.3923/ajb.2015.267.280 DOI: https://doi.org/10.3923/ajb.2015.267.280

Bui A, Sharma R, Henkel R, Agarwal A. Reactive oxygen species impact on sperm DNA and its role in male infertility. Androl. 2018; 50(8):e13012. https://doi.org/10.1111/ and.13012 DOI: https://doi.org/10.1111/and.13012

Hussain T, Kandeel M, Metwally E, Murtaza G, Kalhoro DH, Yin Y, Tan B, Chughtai MI, Yaseen A, Afzal A, Kalhoro MS. Unraveling the harmful effect of oxidative stress on male fertility: A mechanistic insight. Front Endocrinol. 2023; 14:1070692. https://doi.org/10.3389/fendo.2023.1070692 DOI: https://doi.org/10.3389/fendo.2023.1070692

Zhai XW, Zhang YL, Qi Q, Bai Y, Chen XL, Jin LJ, Ma XG, Shu RZ, Yang ZJ, Liu FJ. Effects of molybdenum on sperm quality and testis oxidative stress. Syst Biol Reprod Med. 2013; 59(5):251-5. https://doi.org/10.3109/19396368.2013. 791347 DOI: https://doi.org/10.3109/19396368.2013.791347

Volodymyr IL. Glutathione homeostasis and functions: Potential targets for medical interventions. Journal of Amino Acids. 2012; 2012:736837. https://doi. org/10.1155/2012/736837 DOI: https://doi.org/10.1155/2012/736837

Sasmal N, Kar NC, Mukherjee D, Chatterjee GC. The effect of molybdenum on ascorbic acid metabolism in rats. Biochem J. 1968; 106(3):633–7. https://doi.org/10.1042/ bj1060633 DOI: https://doi.org/10.1042/bj1060633

Foley GL. Overview of male reproductive pathology. Toxicol Pathol. 2001; 29(1):49–63. https://doi. org/10.1080/019262301301418856 DOI: https://doi.org/10.1080/019262301301418856

Guyonnet B, Dacheux F, Dacheux JL, Gatti JL. The epididymal transcriptome and proteome provide some insights into new epididymal regulations. J Androl. 2011; 32:651-64. https://doi.org/10.2164/jandrol.111.013086 DOI: https://doi.org/10.2164/jandrol.111.013086