Development of a New Methodology for Extraction and Analysis of Pesticides, Drugs, Heavy Metals and Various Toxins from Viscera Samples
DOI:
https://doi.org/10.18311/ti/2023/v30i4/35082Keywords:
Drugs, Heavy Metals, Pesticides, Poison, Toxicology, VisceraAbstract
Viscera analysis plays a major and important role in forensics, Scientists use their own tried and tested methods to avoid excess time consumption and errors, to analyse the viscera samples for the presence of various illicit drugs, pesticides, heavy metals, or any other poisonous substances that may be the reason of death. In this article, the current scenario of viscera handling in forensic science laboratories in India is mentioned in detail with a step-by-step procedure. Tests for phosphine and various pesticides are performed in the stomach and small intestine. Drugs are extracted according to different procedures depending upon the affinity of the drug i.e., acidic, basic, or neutral. After that, Tests for drugs and other toxic substances are usually performed in blood, liver, spleen, and kidney. Blood usually comes in the third jar, and is tested for drugs, alcohol, and maybe for pesticides, phosphine and for any other suspected toxin. Viscera sample needs to be digested before the analysis of heavy metals. Any part of the viscera can be digested and tested for heavy metal. Usually, a mixture of all the viscera parts is digested and analysed. The techniques mentioned in this article are rigorously used in the chemistry and toxicology division in forensic laboratories in India.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Rohit Kanojia, A. K. Jaiswal, Arkaja Goswami, Sunita Bhagat
This work is licensed under a Creative Commons Attribution 4.0 International License.
Accepted 2023-10-09
Published 2023-12-11
References
Fischbach P. The role of illicit drug use in sudden death in the young. 2017; 27(S1):S75–9. https://doi.org/10.1017/ S1047951116002274 PMid:28084963 DOI: https://doi.org/10.1017/S1047951116002274
Wilson N, Kariisa M, Seth P, Smith IV H, Davis NL. Drug and opioid-involved overdose deaths- United States, 2017– 2018. Morb Mortal Wkly Rep. 2020; 69(11):290. https://doi. org/10.15585/mmwr.mm6911a4 PMid:32191688 PMCid: PMC7739981 DOI: https://doi.org/10.15585/mmwr.mm6911a4
Eddleston M. Poisoning by pesticides. Medicine (Baltimore) [Internet]. 2020; 48(3):214–7. https://doi.org/10.1016/j. mpmed.2019.12.019 DOI: https://doi.org/10.1016/j.mpmed.2019.12.019
Karunarathne A, Gunnell D, Konradsen F, Eddleston M. How many premature deaths from pesticide suicide have occurred since the agricultural Green Revolution? Clin Toxicol. 2020; 58(4):227–32. https://doi.org/10.1080/15563 650.2019.1662433PMid:31500467 DOI: https://doi.org/10.1080/15563650.2019.1662433
Engwa GA, Ferdinand PU, Nwalo FN, Unachukwu MN. Mechanism and health effects of heavy metal toxicity in humans. Poisoning Mod World-New Tricks Old Dog. 2019; 10:70–90.
Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN. Toxicity, mechanism, and health effects of some heavy metals. Interdiscip Toxicol. 2014; 7(2):60. https://doi.org/10.2478/intox-2014-0009 PMid:26109881 DOI: https://doi.org/10.2478/intox-2014-0009
Gaur JR, Pradesh H. Guiding tips for crime investigation. New Delhi: Government of India; 2014.
Rao PK, Pandey G, Tharmavaram M. Chemical evidence and their handling. In: Technology in Forensic Science. John Wiley & Sons, Ltd; 2020. p. 79–99. https://doi. org/10.1002/9783527827688.ch5 PMid:33321465 DOI: https://doi.org/10.1002/9783527827688.ch5
Rao PK, Pandey G, Tharmavaram M. Biological evidence and their handling. In: Technology in Forensic Science. John Wiley & Sons, Ltd; 2020. p. 35–53. https://doi. org/10.1002/9783527827688.ch3 DOI: https://doi.org/10.1002/9783527827688.ch3
Dinis-Oliveira RJ, Vieira DN, Magalhães T. Guidelines for collection of biological samples for clinical and forensic toxicological analysis. Forensic Sci Res. 2016; 1(1):42–51. https://doi.org/10.1080/20961790.2016.1271098 PMid: 30483610 PMCid: PMC6197137 DOI: https://doi.org/10.1080/20961790.2016.1271098
Jaiswal AK, Choudhary P, Surender DT. Extraction of drugs from biological materials such as viscera, blood, urine and vitreous humour. Int J Med Lab Res. 2018; 2(1):7–14.
Jaiswal AK, Millo T, Murty OP. Extraction, isolation and clean-up methods for pesticides/insecticides and alkaloids from biological material. Part-I. Int J Med Toxicol Leg Med. 2006; 9(1):28–32.
Biswas G. Recent advances in forensic medicine and toxicology. Jaypee Brothers Medical Publishers; 2021. p. 485.
Jaiswal AK, Yadav A, Millo T, Gupta SK. Standard Operating Procedure (SOP) for Microwave Digestion System (MDS- 10). Int J Med Lab Res. 2018; 3(1):49–54.
Bodwal J, Jakhar J. Viscera preservation: Current guidelines and recommendations. Recent Adv Forensic Med Toxicol. 2021; 3:423.
Sharma V. Poisons, viscera analysis, report and its interrelation. Int J Med Toxicol Leg Med. 2004; 6(2):49–54.
Vinoth KV, Selvaraj K, Sudalaimuthu R. A study of autopsy cases - Viscera sample reports a retrospective study. Indian J Forensic Med Toxicol. 2020; 14(4):501–4.
Khan I, Shakeel M, Sharma G. Viscera preservation for postmortem toxicological analysis: A Review. J Indian Acad Forensic Med. 2016; 38(2):241–5. https://doi. org/10.5958/0974-0848.2016.00061.0 DOI: https://doi.org/10.5958/0974-0848.2016.00061.0
Pillay VV, Murty OP, Dikshit PC, Mehrotra VK, Fimate L, Dutta VK, et al. National Committee for revised guidelines of sample preservation and analysis in medico-legal cases. J Forensic Med Toxicol. 1999; 16(1):44–54.
DFSS D of FSS. Updated Working Procedure Manual: Toxicology 2021: All rights reserved with DFSS, MHA, and GoI. Ministry of Home Affairs, GoI; 2021. http://dfs.nic.in/ pdfs/toxicology%20manual%202021.pdf
Bumbrah GS, Krishan K, Kanchan T, Sharma M, Sodhi GS. Phosphide poisoning: A review of the literature. Forensic Sci Int. 2012; 214(1):1–6. https://doi.org/10.1016/j. forsciint.2011.06.018 DOI: https://doi.org/10.1016/j.forsciint.2011.06.018
Raina A, Shrivastava HC, Mathur N, Dogra TD. Validation of qualitative test for phosphine gas in human tissues. IJEB. 2003; 410. http://nopr.niscpr.res.in/handle/123456789/17127
Yadav D, Bhattacharyya R, Banerjee D. Acute aluminium phosphide poisoning: The menace of phosphine exposure. Clin Chim Acta. 2021; 520:34–42. https://doi.org/10.1016/j. cca.2021.05.026PMid:34077754 DOI: https://doi.org/10.1016/j.cca.2021.05.026
Meena MC, Mittal S, Rani Y. Fatal aluminium phosphide poisoning. Interdiscip Toxicol. 2015; 8(2):65–7. https:// doi.org/10.1515/intox-2015-0010 PMid:27486362 PMCid: PMC4961899 DOI: https://doi.org/10.1515/intox-2015-0010
Jayaraj R, Megha P, Sreedev P. Organochlorine pesticides, their toxic effects on living organisms and their fate in the environment. Interdiscip Toxicol. 2016; 9(3–4):90. https:// doi.org/10.1515/intox-2016-0012 PMid:28652852 PMCid: PMC5464684 DOI: https://doi.org/10.1515/intox-2016-0012
Singh Z, Kaur J, Kaur R, Hundal SS. Toxic effects of organochlorine pesticides: A review. Am J BioSci. 2016; 4(3):11. https://doi.org/10.11648/j.ajbio.s.2016040301.13 DOI: https://doi.org/10.11648/j.ajbio.s.2016040301.13
Eto M. Organophosphorus pesticides. CRC Press; 2018.
Kuhr RJ, Dorough HW. Carbamate insecticides: Chemistry, biochemistry, and toxicology. CRC Press, Inc; 1976.
Bradberry SM, Cage SA, Proudfoot AT, Vale JA. Poisoning due to pyrethroids. Toxicol Rev. 2005; 24:93–106. https://doi.org/10.2165/00139709-200524020-00003 PMid:16180929 DOI: https://doi.org/10.2165/00139709-200524020-00003
Jaiswal AK, Millo T. Handbook of forensic analytical toxicology. Jaypee Brothers Medical Publishers; 2022.
Curry A. Toxicological Analysis. J Pharm Pharmacol. 2011; 12(1):321–39. https://doi.org/10.1111/j.2042-7158.1960. tb12687.x DOI: https://doi.org/10.1111/j.2042-7158.1960.tb12672.x