Effect of Iron Overload on Tight Junctions and Adhesion Molecules in ECV304 Cells

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Authors

  • Samed Refik Sar
     Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Uskudar University, Istanbul - 34662 ,TR
  • Furkan Meric
     Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Uskudar University, Istanbul - 34662 ,TR
  • Aysegul Yanik Ilgar
     Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Uskudar University, Istanbul - 34662 ,TR
  • Irem Gulfem Albayrak
     Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Uskudar University, Istanbul - 34662 ,TR
  • Belkis Atasever Arslan
     Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Uskudar University, Istanbul - 34662 ,TR

DOI:

https://doi.org/10.18311/ti/2024/v31i3/35986

Keywords:

Blood- Brain Barrier, ECV-304, Gene Expression Analysis, Iron Overload

Abstract

Blood vessels are essential for the body’s tissues and organs to receive oxygen and nutrition. The Blood-Brain Barrier (BBB) is a special feature of the blood arteries that vascularise the Central Nervous System (CNS) which enables these vessels to tightly control the flow of ions and molecules between the blood and the brain. The accurate regulation of CNS homeostasis facilitates appropriate neuronal performance and safeguards neural tissue from toxins and pathogens. Modifications to these barrier characteristics play a significant role in the development of many neurological disorders. BBB contains tight junction transmembrane proteins, integral membrane proteins, occludin, claudins, IgG-type proteins, junction adhesion molecules and scaffold proteins. Numerous biological functions require iron in the CNS, including neurotransmitter synthesis, myelin formation and mitochondrial function. However, excess iron can lead to oxidative stress and damage, which are implicated in neurodegenerative diseases. In this research, the impacts of iron accumulation on Cadherin 5 (CDH5), Claudin 5 (CLDN5), Intercellular Adhesion Molecule 1 (ICAM-1), Occludin (OCL), p-selectin (P-SEL), Vascular Cell Adhesion Molecule 1 (VCAM-1) and Zonula occludens-1 (ZO-1) genes expressions in Human Umbilical Vein Endothelial Cells (ECV 304) cells were investigated. It was found that in human umbilical vein endothelial cell line cells, iron overload enhanced the expression of CDH5 and P-SEL genes while reducing the expression of VCAM1, Cldn5, ICAM-1, OCL, and Zo-1 genes.

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Published

2024-06-10

How to Cite

Sar, S. R., Meric, F., Yanik Ilgar, A., Albayrak, I. G., & Arslan, B. A. (2024). Effect of Iron Overload on Tight Junctions and Adhesion Molecules in ECV304 Cells. Toxicology International, 31(3), 429–434. https://doi.org/10.18311/ti/2024/v31i3/35986

Issue

Volume 31, Issue 3, July-September 2024

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Articles

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Copyright (c) 2024 Samed Refik Sar, Furkan Meric, Aysegul Yanik Ilgar, Irem Gulfem Albayrak, Belkis Atasever Arslan

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Received 2023-12-26
Accepted 2024-02-23
Published 2024-06-10

 

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