Exploring eco-sensitive strategies for effective powdery mildew management in grapevines

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Authors

  • RANJAN KUMAR JENA
     Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai – 625104, Tamil Nadu ,IN
  • I. YESU RAJA
     Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai – 625104, Tamil Nadu ,IN
  • V. RAMAMOORTHY
     Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Eachangkottai, Thanjavur – 614902, Tamil Nadu ,IN
  • S. LAKSHMI NARAYANAN
     Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai – 625104, Tamil Nadu ,IN
  • R. RENUKA
     Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai – 625104, Tamil Nadu ,IN
  • A. SUBBIAH
     Grape Research Station, Anaimalayanpatty, Tamil Nadu Agricultural University, Theni – 625526, Tamil Nadu ,IN
  • K. ERAIVAN ARUTKANI AIYANATHAN
     Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai – 625104, Tamil Nadu ,IN
  • K. ERAIVAN ARUTKANI AIYANATHAN
     Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai – 625104, Tamil Nadu ,IN
  • V. KARTHIK PANDI
     Grape Research Station, Anaimalayanpatty, Tamil Nadu Agricultural University, Theni – 625526, Tamil Nadu ,IN
  • R. SIVADHARSHANAPRIYA
     Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai – 625104, Tamil Nadu ,IN

DOI:

https://doi.org/10.18311/jbc/2023/34206

Keywords:

Ampelomyces quisqualis, compatibility, GC-MS, hyperparasitism, powdery mildew, Trichoderma asperellum

Abstract

Grapevine powdery mildew is one of the most important plant diseases widely affecting crops in many countries. The main aim of the present study was to use Ampelomyces quisqualis isolates to suppress the powdery mildew of grapes under field conditions. The findings revealed a strong correlation between the mycoparasite, Ampelomyces quisqualis and the grape powdery mildew pathogens, suggesting its potential for effective control. The results revealed that a single spray of A. quisqualis @ 2.0 per cent (MDU1) succeeded by two sprays of Trichoderma asperellum @ 2.0 per cent (Tasp,7) were found to be effective against Erysiphe necator. In addition, liquid formulation of A. quisqualis isolate MDU1 (2% w/v) as a foliar spray at 2% containing 2 × 106 spores ml-1 the during initial occurrence of the disease up to three sprayings at 15 days interval was found to be best. Thus, a liquid formulation of A. quisqualis isolate MDU1 proved effective in managing grapes powdery mildew disease under field conditions. The rDNA ITS region of A. quisqualis isolates was analyzed at the molecular level, and the resulting sequences were subjected to GC-MS analysis. The secondary metabolite identification using GC-MS revealed the presence of antimicrobial compounds, including squalene with the highest peak of 4.643 percent, octadecanoic acid with 3.862 percent, tetradecanoic acid with 3.600 percent, and 9,12-octadecadienoic acid (Z,Z) with 1.451 percent. These bioactive compounds revealed by GC-MS analysis in crude extracts of A. quisqualis had a stronger antifungal activity against E. necator.

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Published

2023-08-23

How to Cite

JENA, R. K., RAJA, I. Y., RAMAMOORTHY, V., S. LAKSHMI NARAYANAN, RENUKA, R., A. SUBBIAH, K. ERAIVAN ARUTKANI AIYANATHAN, K. ERAIVAN ARUTKANI AIYANATHAN, V. KARTHIK PANDI, & R. SIVADHARSHANAPRIYA. (2023). Exploring eco-sensitive strategies for effective powdery mildew management in grapevines. Journal of Biological Control, 37(1), 32–45. https://doi.org/10.18311/jbc/2023/34206

Issue

Volume 37, Issue 1, March 2023

Section

Research Articles

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Copyright (c) 2023 RANJAN KUMAR JENA, I. YESU RAJA, V. RAMAMOORTHY, S. LAKSHMI NARAYANAN, R. RENUKA, A. SUBBIAH, K. ERAIVAN ARUTKANI AIYANATHAN, K. ERAIVAN ARUTKANI AIYANATHAN, V. KARTHIK PANDI, R. SIVADHARSHANAPRIYA

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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Received 2023-06-24
Accepted 2023-07-31
Published 2023-08-23

 

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