Alleviating Potential of Zingiber officinale and Cow Urine Distillate Co-administered with Levetiracetam in Epileptic Rats: A Pharmacokinetic and Pharmacodynamics Approach

study of Levetiracetam in pilocarpine-induced epilepsy

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Authors

  • Nilay Solanki
     Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa – 388421, Gujarat ,IN
  • Harshal Patel
     Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa – 388421, Gujarat ,IN
  • Milap Patel
    Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa – 388421, Gujarat ,IN
  • Yamini Patel
     Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa – 388421, Gujarat ,IN
  • Preetu Shukla
     Department of Pharmacology, Parul Institute of Pharmacy and Research, Parul University, Vadodara – 391350, Gujarat ,IN
  • Jagdish Kakadiya
     Department of Pharmacology, Parul Institute of Pharmacy and Research, Parul University, Vadodara – 391350, Gujarat ,IN
  • Rajesh Maheshwari
     Department of Pharmacy, Sumandeep Vidyapeeth Deemed to be University, Piparia, Vadodara – 391760, Gujarat ,IN
  • Priyal Chauhan
     Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa – 388421, Gujarat ,IN

DOI:

https://doi.org/10.18311/jnr/2023/33217

Keywords:

Catalase and Nitrate, Cow Urine Distillate, Epilepsy, Levetiracetam, Zingiber officinale Extract

Abstract

Background: Epilepsy is a severe neurological condition that affects all ages of people. Complex pathways involved in pathogenesis make it complicated to treat; selected antiepileptic drug options are available for Epilepsy. There is a need for an hour to develop novel treatment approaches for epilepsy with lesser side effects. This research aimed to evaluate the alleviating role of bio-enhancers co-administered with levetiracetam for pilocarpine-induced epilepsy. Methodology: Pilocarpine (250 mg/kg) was used to develop epilepsy in rats. Levetiracetam (LEV) (140 mg/kg) was administered with Zingiber officinale Extract (ZOE) (15 mg/kg and 30 mg/kg) and Cow Urine Distillate (CUD) (1.5 ml/kg and 3 ml/kg). HPLC was used to evaluate drug concentration in blood. Serum nitrate, catalase, CRP, calcium level and calcium level of the brain, behavioural markers in rats were assessed and compared with the Leviteracetam group only. Result and Discussion: The present study showed that combining ZOE and CUD with levetiracetam was advantageous through substantial reduction (p < 0.05) in serum nitrate, CRP and increased catalase (p < 0.05), while reduced serum calcium compared to LEV alone. Combination of ZOE and CUD with levetiracetam treatment also reduced seizure behaviour and duration in rats. The bioavailability of LEV in plasma and brain was increased when epileptic rats were treated with LEV plus ZOE and CUD compared to disease control. Conclusion: Utilization of Zingiber officinale and CUD in combination with LEV was proven therapeutically effective in the epileptic model and used to lower the dose of LEV along with reducing seizure behaviour and time with the potential for the treatment of epilepsy.

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Author Biographies

Nilay Solanki, Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa – 388421, Gujarat

Dr. Nilay is an Associate Professor at Ramanbhai Patel College of Pharmacy, CHARUSAT, India.
He studied Masters in Pharmacology at LMCP, India and completed his Ph.D. in the year
2016 from CHARUSAT University, India. He joined the Ramanbhai Patel College of
Pharmacy, CHARUSAT and developed his research core in the area of diabetic complications
and neuropharmacology. His expertise is in the area of clinical research and preclinical
animal model development of various disease conditions special emphasis on diabetes,
NAFLD, obesity, Neurodegeneration etc. He has published more than 40 research review
papers from Scopus and web of science listed journals and completed consultancy projects
worth 0.4 million INR. He had received various awards in national conferences and also
provided his services as a resource person in national and international conferences in
India.

 

 

Harshal Patel, Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa – 388421, Gujarat

Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa - 388421, Gujarat, India

Milap Patel , Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa – 388421, Gujarat

Assistant professor at Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa - 388421, Gujarat, India

Yamini Patel, Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa – 388421, Gujarat

M.Pharm (Pharmacology) student at Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa - 388421, Gujarat, India

Preetu Shukla, Department of Pharmacology, Parul Institute of Pharmacy and Research, Parul University, Vadodara – 391350, Gujarat

Ph.D student at CHARUSAT university, changa

 

 

Jagdish Kakadiya, Department of Pharmacology, Parul Institute of Pharmacy and Research, Parul University, Vadodara – 391350, Gujarat

Associate professor at Department of Pharmacology, Parul Institute of Pharmacy and Research, Parul University, Vadodara - 391350, Gujarat, India

 

 

Rajesh Maheshwari, Department of Pharmacy, Sumandeep Vidyapeeth Deemed to be University, Piparia, Vadodara – 391760, Gujarat

Professor at Department of Pharmacy, Sumandeep Vidyapeeth Deemed to be University, Piparia, Vadodara – 391760, Gujarat, India. Over 15 years of teaching and research experience.

 

Priyal Chauhan, Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT-Campus, Changa – 388421, Gujarat

 

 

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Published

2023-06-13

How to Cite

Solanki, N., Patel, H., Patel , M., Patel, Y., Shukla, P., Kakadiya, J., Maheshwari, R., & Chauhan, P. (2023). Alleviating Potential of <i>Zingiber officinale</i> and Cow Urine Distillate Co-administered with Levetiracetam in Epileptic Rats: A Pharmacokinetic and Pharmacodynamics Approach: study of Levetiracetam in pilocarpine-induced epilepsy. Journal of Natural Remedies, 23(2), 677–685. https://doi.org/10.18311/jnr/2023/33217

Issue

Volume 23, Issue 2, April 2023

Section

Short Communication

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Copyright (c) 2023 Nilay Solanki, Harshal Patel, Milap Patel , Yamini Patel, Preetu Shukla, Jagdish Kakadiya, Rajesh Maheshwari, Priyal Chauhan (Author)

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Received 2023-03-02
Accepted 2023-04-24
Published 2023-06-13

 

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