Studies on Apoptotic Changes in Combined Toxicity of Citrinin and Endosulfan in Pregnant Wistar Rats and Their Fetuses

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Authors

  • N. D. Singh
     COVS, GADVASU, Ludhiana ,IN
  • A. K. Sharma
     Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh ,IN
  • P. K. Dwivedi
     Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh ,IN
  • A. G. Telang
     CADRAD, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh ,IN
  • M. Kumar
     HSADL, Bhopal, Madhya Pradesh ,IN
  • R. D. Patil
     Department of Veterinary Pathology, GC Negi COVS, CSKHPKVV, Palampur, Himachal Pradesh ,IN

Keywords:

Apoptosis, citrinin, endosulfan, fetuses, pregnant dams
Neuroethology

Abstract

Background: Citrinin (mycotoxin) and endosulfan (pesticide) both environmental contaminants easily enter the food chain and are caoomon causes of various toxicities. Materials and Methods: In the present investigation, citrinin (CIT) (10 mg/kg feed) and endosulfan (1 mg/kg body weight) were administered orally alone and in combination to pregnant Wistar rats from gestational day 6 to 20 to study their effect to cause apoptosis in the pregnant Wistar rats and their fetuses. Apoptosis was assessed in dams by agarose gel electrophoresis, flow cytometry and electron microscopy, while in the fetuses it was assessed by flow cytometry only. Result: Citrinin and endosulfan in the combination group caused apoptosis in an additive manner as there was increased number of apoptotic cells as compared to the individual toxin and control groups. The fetuses also showed increased number of apoptotic cells in the combination groups, which also indicated that both the toxins crossed the placental barrier. Conclusion: So it was concluded that apoptosis played a significant role in the pathogenesis of endosulfan and citrinin toxicity.

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Published

2018-05-25

How to Cite

Singh, N. D., Sharma, A. K., Dwivedi, P. K., Telang, A. G., Kumar, M., & Patil, R. D. (2018). Studies on Apoptotic Changes in Combined Toxicity of Citrinin and Endosulfan in Pregnant Wistar Rats and Their Fetuses. Toxicology International, 19(2), 138–143. Retrieved from https://informaticsjournals.co.in/index.php/toxi/article/view/21355

Issue

Volume 19, Issue 2, May-August 2012

Section

Original Research
Received 2018-05-24
Accepted 2018-05-24
Published 2018-05-25

 

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