Bio-Antioxidants Protect the Buffalo Bone Marrow Derived Mesenchymal Stem Cells against Oxidative Stress Induced During Freeze-Thaw Cycle
Authors
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Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab ,IN
Navjot Kaur -
Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab ,INM. Sharma
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Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab ,INM. K. Lonare
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Department of Veterinary Surgery and Radiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab ,INR. Udehiya
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Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab ,IND. Singh
DOI:
https://doi.org/10.18311/ti/2021/v28i1/24809Keywords:
Antioxidants, Buffalo, Curcumin, Mesenchymal Stem Cells, Oxidative Stress, ResveratrolAbstract
The present investigation was planned to evaluate the effect of curcumin (CUR) and resveratrol (RES) on oxidative stress indicators (LPO, O2- radical, ROS and TPC), antioxidant status (GSH, GPx, SOD, CAT and GST) and cytotoxicity markers (ALP, LDH, GGT and CK-MB) in mesenchymal stem cells (MSCs) derived from buffalo bone marrow during the freeze-thaw cycle of one month. MSCs were isolated and cultured in high glucose DMEM supplemented with 15% FBS. MSCs were found to be positive for alkaline phosphatase (AP) activity and stem cell markers (CD73 and OCT4). Results revealed a significant reduction in (p≤0.05) in oxidative stress parameters and cytotoxicity markers, while, the levels of cellular antioxidants were found to be significantly increased (p≤0.05) in CUR/RES alone and in combination groups as compared to the control group. In conclusion, the addition of bioantioxidants in the cryopreservation medium has improved the post-thaw cell recovery through suppression of oxidative stress-induced during the freeze-thaw cycle.
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Accepted 2020-11-04
Published 2021-03-24
References
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