Amelioration of Streptozotocin Induced Cytotoxicity by Mimosa pudica Root Extract - A Proteomic Based In-Vitro Study

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Authors

  • Pallavi S. Jamadagni
     Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra ,IN
  • Sharad D. Pawar
     Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra ,IN
  • Shrirang Jamadagni
     Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra ,IN
  • Shridhar Chougule
     Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra ,IN
  • Manish Wanjari
     Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra ,IN
  • Manish Gautam
     Assam Down Town University, Panikhaiti, Guwahati - 781026, Assam ,IN
  • Sudesh Gaidhani
     Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, 61-65, Institutional Area, Opposite Janakpuri D Block, New Delhi - 110058 ,IN
  • Arun Gurav
     Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra ,IN
  • N. Shrikanth
     Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, 61-65, Institutional Area, Opposite Janakpuri D Block, New Delhi - 110058 ,IN

DOI:

https://doi.org/10.18311/ti/2022/v29i4/29645

Keywords:

LC-MS, Mimosa pudica, Proteomics, RIN5F.

Abstract

The in-vitrocytotoxicity was studied in a pancreatic cell line (RIN5F) using streptozotocin-induced cellular damage. The Cytotoxic dose of aqueous and hydro-alcoholic extracts of Mimosa pudica root powder before and after streptozotocin treatment was studied and protein expression was explored. Both the extracts were subjected to metabolite analysis by LCMS. Mimosa pudica root powder extracts prevented Streptozotocin-induced cellular damage. Proteomics studies indicated both aqueous and hydro-alcoholic extracts of Mimosa pudica regulate proteins related to insulin secretion, glucose uptake, and insulin resistance with marked changes in proteins involved in insulin secretion.  The study first time reports cytotoxicity of Mimosa pudica in RIN5F cells and explores protein expression after Mimosa pudica treatment. It was also observed that the abundance of Mimosine is more in Hydro-alcoholic extracts as compared to aqueous extracts. 

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Published

2023-02-07

How to Cite

Jamadagni, P. S., Pawar, S. D., Jamadagni, S., Chougule, S., Wanjari, M., Gautam, M., Gaidhani, S., Gurav, A., & Shrikanth, N. (2023). Amelioration of Streptozotocin Induced Cytotoxicity by <i>Mimosa pudica</i> Root Extract - A Proteomic Based In-Vitro Study. Toxicology International, 29(4), 447–454. https://doi.org/10.18311/ti/2022/v29i4/29645

Issue

Volume 29, Issue 4, December 2022

Section

Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2022-02-24
Accepted 2022-04-30
Published 2023-02-07

 

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