Protective Effect of Pomegranate Juice Extract Against Antitubercular Drugs Induced Hepatic Fibrosis

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Authors

  • J. Ramesh
     Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad - 500030, Telangana ,IN
  • Anilkumar Banothu
     Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad - 500030, Telangana ,IN
  • Kala Kumar Bharani
     Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad - 500030, Telangana ,IN
  • P. Shivakumar
     Departmen of Veterinary Pharmacology and Toxictology, College of Veterinary Science, Rajendranagar, Hyderabad - 500030, Telangana ,IN
  • M. Jeevanalatha
     Department of Pathology, College of Veterinary Science, Mamanoor, Warangal - 506166, Telangana ,IN
  • Y. Ravikumar
     Department of Pathology, College of Veterinary Science, Rajendranagar, Hyderabad - 500030, Telangana ,IN

DOI:

https://doi.org/10.18311/ti/2023/v30i3/30923

Keywords:

Antioxidants, Antitubercular Drugs, Hepatotoxicity, Pomegranate Juice, TGF Beta
pharmacology and toxicology

Abstract

The current study aimed to know the therapeutic efficacy of Punica granatum (fruit juice extract) against anti-TB druginduced hepatic fibrosis in rats. 24 male Wistar albino rats were randomly divided into four groups, with six rats in each. INH at 27 mg/kg B.W., RIF at 54 mg/kg B.W., and PZA at 135 mg/kg B.W. were administered orally to Groups 2, 3 and 4 from days 1 to 28. Group 1 was maintained as normal control, and group 2 was a toxic control (administered anti-TB drugs, p/o). Groups 3 and 4 were administered (p/o) with Enalapril at 5mg/kg B.W. and Punica granatum (fresh juice extract) at 1 ml/rat, respectively, from day 1 to 28. Serum samples were collected on the 14th and 28th to assess various biochemical parameters, and liver samples were collected at the end of the experiment to analyze antioxidant parameters, TGF β1, and histopathological examination. The biochemical parameters showed significant (p<0.05) alterations in AST, ALT, GGT, antioxidant profile (TBARS GSH, GST, GPx and SOD), and expression of TGF β1 levels in group 2 along with collagen deposition and alterations in the histopathology when compared with group 1. There was a significant improvement in treatment groups 3 and 4 at different time intervals. These changes were reversed in groups 3 and 4, which were administered Enalapril and Punica granatum juice extract, respectively.

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Published

2023-09-20

How to Cite

Ramesh, J., Banothu, A., Bharani, K. K., Shivakumar, P., Jeevanalatha, M., & Ravikumar, Y. (2023). Protective Effect of Pomegranate Juice Extract Against Antitubercular Drugs Induced Hepatic Fibrosis. Toxicology International, 30(3), 309–316. https://doi.org/10.18311/ti/2023/v30i3/30923

Issue

Volume 30, Issue 3, July-September 2023

Section

Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2022-08-16
Accepted 2023-06-30
Published 2023-09-20

 

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