DNA Protective, Aldose Reductase Inhibitory and Anti-cataract Activities of Ferulic Acid

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Authors

  • Nagaraju Bandaru
     1School of Pharmaceutical Sciences (SOPS), Sandip University, Nasik - 422213, Maharashtra, India ,IN
  • Veera Mani Deepika Pedapati
     3Department of Pharmacy Practice, Aditya Pharmacy College, Surampalem, Kakinada – 533437, Andhra Pradesh ,IN
  • Matta Sarika
     Department of Pharmacy Practice, Aditya Pharmacy College, Surampalem, Kakinada – 533437, Andhra Pradesh ,IN
  • Suggu Vangmai Swaroopa
     Department of Pharmacy Practice, Aditya Pharmacy College, Surampalem, Kakinada – 533437, Andhra Pradesh ,IN
  • M. Aparna
     College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddesweram – 522302, Andhra Pradesh ,IN
  • Md. Said Ibrahim
     College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddesweram – 522302, Andhra Pradesh ,IN
  • Makarand Suresh Gambhire
     School of Pharmaceutical Sciences (SOPS), Sandip University, Nasik - 422213, Maharashtra ,IN
  • Ramu Samineni
     School of Pharmaceutical Sciences (SOPS), Sandip University, Nasik - 422213, Maharashtra ,IN
  • M. Narender
     College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddesweram – 522302, Andhra Pradesh ,IN
  • Prashik B. Dudhe
     School of Pharmaceutical Sciences (SOPS), Sandip University, Nasik - 422213, Maharashtra ,IN

Keywords:

Antioxidants, DNA, Docking, Diabetes, Ferulic Acid, Glucose Concentrations

Abstract

The current study assessed Ferulic acid’s DNA protective, aldose reductase inhibitory and anti- cataract activities. Different concentrations of ferulic acid are examined for DNA protection and incubated with aldose reductase isolated from a goat lens. Ferulic acid demonstrates a strong inhibitory effect against aldose reductase enzyme with IC50 value of 1.43 g/ mL and shows potent DNA protective action at the dose of 100 ug/mL against Fenton’s reagent induced DNA damage. Ferulic acid also reduces the formation of opacification in goat lens that inhibits the development of cataract and increases antioxidants levels significantly in lenses. This indicated that Ferulic acid inhibits the oxidative stress induced cataract. Based on the findings, Ferulic acid may be an effective option for the treatment of high glucose-induced cataract.

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Published

2024-08-23

How to Cite

Bandaru, N., Pedapati, V. M. D., Sarika, M., Swaroopa, S. V., Aparna, M., Ibrahim, M. S., Gambhire, M. S., Samineni, R., Narender, M., & Dudhe, P. B. (2024). DNA Protective, Aldose Reductase Inhibitory and Anti-cataract Activities of Ferulic Acid. Toxicology International. Retrieved from https://informaticsjournals.co.in/index.php/toxi/article/view/43004

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Online First

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Copyright (c) 2024 Nagaraju Bandaru, Veera Mani Deepika Pedapati, Matta Sarika, Suggu Vangmai Swaroopa, M. Aparna, Md. Said Ibrahim, Makarand Suresh Gambhire, Ramu Samineni, M. Narender, Prashik B. Dudhe

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Received 2024-03-22
Accepted 2024-06-21
Published 2024-08-23

 

References

Imran A, Shehzad MT, Shah SJA. et al. Development and exploration of novel substituted thiosemicarbazones as inhibitors of aldose reductase via in vitro analysis and computational study. Sci Rep. 2022. https://doi.org/10.1038/s41598-022-09658-z

Yu MG, Gordin D, Fu J, Park K, Li Q, King GL. Protective factors and the pathogenesis of complications in diabetes. End Rev. 2024; 45(2):227–252. https://doi.org/10.1210/endrev/bnad030

Shahab M, Zheng G, Alshabrmi FM, Bourhia M, Wondmie GF, Salamatullah MA. Exploring potent aldose reductase inhibitors for anti-diabetic (anti-hyperglycemic) therapy: integrating structure-based drug design, and MMGBSA approaches. Front Mol Biosci. 2023; 20(10). https://doi. org/10.3389/fmolb.2023.1271569

Mrugacz M, Pony-Uram M, Bryl A, Zorena K. Current approach to the pathogenesis of diabetic cataracts. Int J Mol Sci. 2023; 24(7):6317. https://doi.org/10.3390/ijms24076317

Zhu C. Aldose reductase inhibitors as potential therapeutic drugs for diabetic complications. Diabetes Mellitus - Insights and Perspectives. 2013. https://doi.org/10.5772/54642

Kador PF, Wyman M, Oates PJ. Aldose reductase, ocular diabetic complications and the development of topical Kinostat(®). Prog Retin Eye Res. 2016; 54:1-29. https://doi. org/10.1016/j.preteyeres.2016.04.006

Mrugacz M, Pony-Uram M, Bryl A, Zorena K. Current approach to the pathogenesis of diabetic cataracts. International Journal of Molecular Sciences. 2023; 24(7):6317. https://doi.org/10.3390/ijms24076317

Snow A, Shieh B, Chang KC, Pal A, Lenhart P, Ammar D, Ruzycki P, Palla S, Reddy GB, Petrash JM. Aldose reductase expression as a risk factor for cataract. Chem Biol Interact. 2015; 234:247-253. PMID: 25541468. https://doi. org/10.1016/j.cbi.2014.12.017.

Robert S, Randall LK, editors. Cataract Overview (Internet). Life Extension. Available from: http://www.garynullforum. com/Articles/pdf/foundarticles1/lef/CATARACTS.pdf

Obrosova IG, Chung SS, Kador PF. Diabetic cataract: Mechanisms and management. Diabetes Metab Res Rev 2010; 26:172-180. https://doi.org/10.1002/dmrr.1075

Dongare V, Kulkarni C, Kondawar M, Magdum C, Haldavnekar V, Arvindekar A. Inhibition of aldose reductase and anti-cataract action of trans-anethole isolated from Foeniculum vulgare Mill. Fruits Food Chem. 2012;132(1):385-390. https://doi.org/10.1016/j. foodchem.2011.11.005

Mattila P, Kumpulainen J. Determination of free and total phenolic acids in plant- derived foods by HPLC with diode-array detection. J Agric Food Chem. 2002; 50(13):3660–3667. https://doi.org/10.1021/jf020028p

Devi VS, Vellapandian C, Ilavarasan R, Sumithra M. Role of phytochemicals against diabetic nephropathy: An insight into molecular receptors. Toxicol Int. 2023; 30(4):585– 604. https://doi.org/10.18311/ti/2023/v30i4/30998

Lee JC, Kim HR, Kim J, Jang YS. Antioxidant property of an ethanol extract of the stem of Opuntia ficus-indica var. Saboten. J Agric Food Chem. 2002; 50(22):6490– 6496. https://doi.org/10.1021/jf020388c

Saraswat M, Muthenna P, Suryanarayana P, Petrash JM, Reddy GB. Dietary sources of aldose reductase inhibitors: Prospects for alleviating diabetic complications. Asia Pac J Clin Nutr. 2008; 17(4):558-565.

Somani G, Sathaye S. Bioactive fraction of Saraca indica prevents diabetes-induced cataractogenesis: Aldose reductase inhibitory activity. Pharmacogn Mag. 2015; 11(41):102-110. https://doi.org/10.4103/0973-1296.149722

Arumugam M, Muthuswamy U, Kuppusamy A, Thirumalaisamy S, Varadharajan S, Puliyath J. Discovery of potential aldose reductase inhibitors using in silico docking studies. Orient Pharm Exp Med. 2012; 12:157-1 61. https:// doi.org/10.1007/s13596- 012-0065-3

Bandaru N, Ramu A, Vijetha P, Vidhyadhara S, Nandini P, Reddy AS. Evaluation of ex vivo anti-cataract activity of ethanolic extract of Alstonia scholaris leaves on dexamethasone-induced cataract using isolated goat lens. Asian J Pharm Clin Res. 2017; 10(2):182-185. https://doi. org/10.22159/ajpcr.2017.v10i2.15084

Estimation of total protein by the modified biuret endpoint method. Available from: http://www. btprotocolsmaulikblogspot. in/2007/04/estimateprotein- by.html.

Weydert CJ, Cullen JJ. Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nat Protoc. 2009; 5(1):51-66. https://doi. org/10.1038/nprot.2009.197

Muthenna RP, Akileshwari C, Saraswat M, Petrash JM. Inhibition of aldose reductase and sorbitol accumulation by dietary rutin. Curr Sci. 2011; 101(9):1191-1197.

Ganeshpurkar A, Bhadoriya SS, Pardhi P, Jain AP, Rai G. In vitro prevention of cataract by Oyster Mushroom Pleurotus florida extract on isolated goat eye lens. Indian J Pharmacol. 2011; 43(6):667-670. https://doi.org/10.4103/0253-7613.89823 23. Giblin FJ. Glutathione: A vital lens antioxidant. J Ocul Pharmacol Ther. 2000; 16(2):121-135. https://doi. org/10.1089/jop.2000.16.121