Ameliorative Effects of Operculina turpethum and its Isolated Stigma"‘5,22dien"‘3-o-b-D-glucopyranoside on the Hematological Parameters of Male Mice Exposed to N"‘Nitrosodimethylamine, a Potent Carcinogen

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Authors

  • Veena Sharma
     ,IN
  • Manu Singh
     ,IN

Keywords:

Blood, haematology, N"‘Nitrosodimethylamine, Operculina turpethum

Abstract

Objectives: Enormous propensity of plants to synthesize a variety of structurally diverse bioactive compounds, has made the plant kingdom a potential source of chemical constituents with various therapeutic values, including antitumor and cytotoxic activities. Blood is a good indicator to determine the physiological and pathological status of man and animal. The objective of the present study is to determine the effect of Operculina turpethum root extract and its isolated glycoside treatment on the hematological parameters in the mice with N"‘Nitrosodimethylamine (NDMA) induced cancer. Materials and Methods: The body weights of the animals were recorded before and after the experiment. Non"‘coagulated blood was tested for total erythrocyte count, total leukocyte count, hemoglobin, differential leukocyte count (DLC) and for other blood indices. Results: A significant (P < 0.01), (P < 0.001) recovery of the red blood cell and white blood cell counts, packed cell volume and hemoglobin content in the host after 21 day treatment was shown. Conclusion: These results show that the extract of Operculina turpethum is relatively safe following oral administration and have possible stimulatory effect on red blood cell production and there was dose dependent therapeutic effect.

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Published

2018-04-25

How to Cite

Sharma, V., & Singh, M. (2018). Ameliorative Effects of Operculina turpethum and its Isolated Stigma"‘5,22dien"‘3-o-b-D-glucopyranoside on the Hematological Parameters of Male Mice Exposed to N"‘Nitrosodimethylamine, a Potent Carcinogen. Toxicology International, 21(1), 29–36. Retrieved from https://informaticsjournals.co.in/index.php/toxi/article/view/20956

Issue

Volume 21, Issue 1, January-April 2014

Section

Original Research
Received 2018-04-20
Accepted 2018-04-20
Published 2018-04-25

 

References

Kala SM, Tresina Soris P, Mohan VR. Antitumour activity of Eugenia flocossa Bedd and Eugenia singampattiana Bedd leaves against Dalton ascites lymphoma in Swiss albino rats. Int J Pharm Tech Res 2011;3:1796"‘800.

Sangameswaran B, Saluja MS, Sharma A. Anticancer activity of ethanol extract of Madhuca longifolia against Ehrlich Ascites Carcinoma. Mol Clin Pharmacol 2012;2:12"‘9.

Xia M, Wang D, Wang M, Tashiro S, Onodera S, Minami M, et al. Dracorhodin perchocolate induces apoptosis via activation of caspases and generation of reactive oxygen species. J Pharmacol Sci 2004;95:273"‘83.

Cheeke PR, Shull LR. Natural toxicants in feeds and poisonous plants. New York: AVI Publishing; 1999. p. 34"‘7.

Austin CC, Perkins SL. Parasites in a biodiversity hotspot: A survey of hematozoa and a molecular phylogenetic analysis of Plasmodium in New Guinea skinks. J Parasitol 2006;92:770"‘7.

Coles EH. Veterinary Clinical Pathology. Philadelphia: W.B.Saunders Company; 1986. p. 17"‘9.

Sharma V, Singh M. Alterations induced by N"‘Nitrosodimethylamine and ethanolic root extract of Operculina turpethum in serum lipid profile of male albino mice. Asian J Pharm Clin Res 2012;5:69"‘73.

Sharma V, Singh M. Operculina turpethum as a panoramic herbal medicine. Int J Pharm Sci Res 2012;3:1"‘5.

Sharma V, Singh M. In vitro radical scavenging activity and phytochemical screening for evaluation of the antioxidant potential of Operculina turpethum root extract. J Pharm Res 2012;5:783"‘7.

Pamela C, Chanpe RA, Harvey JB. Lippincott's Illustrated reviews. Biochemistry. 2nd ed. Philadelphia: Lippincott Company; 1994. p. 306.

Ling WH, Jones PJ. Dietary phytosterols: A review of metabolism, benefits and side effects. Life Sci 1995;57:195"‘206.

Pegel KH. The importance of sitosterol and sitosterolin in human and animal nutrition. South Afr J Sci 1997;93:263"‘8.

Moreau RA, Whitaker BD, Hicks KB. Phytosterols, phytostanols, and their conjugates in foods: Structural diversity, quantitative analysis, and health"‘promoting uses. Prog Lipid Res 2002;41:457"‘500.

Bouic PJ. The role of phytosterols and phytosterolins in immune modulation: A review of the past 10 years. Curr Opin Clin Nutr Metab Care 2001;4:471"‘5.

Hogland HC. Haematological complications of cancer chemotherapy. Semin Oncol 1982;9:95"‘102.

Fenninger LD, Mider GB. Energy and nitrogen metabolism in cancer. In: Greenstein JP, Haddow A, editors. Advances in Cancer Research. Vol. 2. New York: Academic Press Inc; 1954. p. 229"‘53.

Cynthia CC, Berger BJ. Laboratory Tests and Diagnostic Procedures. 3rd ed. Philadelphia: W. B. Saunders Company; 2001.

Sharma V, Singh M. N"‘Nitrosodimethylamine as a hazardous chemical toxicant in drinking water. Int Res J Pharm 2012;3.

Biaudet H, Mavelle T, Debry G. Mean daily intake of N"‘nitrosodimethylamine from foods and bevearages in France in 1987 − 1992. Food Chem Toxicol 1994;32:417"‘21.

Hamilton MA, Rosemarie CR, Robert VT. Trimmed Spearman"‘Karber Method for estimating median lethal concentrations in toxicity bioassays. Environ Sci Tech 1977;11:714"‘8.

Ahmad R, Ahmed S, Khan NU, Hasnain AU. Operculina turpethum attenuates N"‘nitrosodimethylamine induced toxic liver injury and clastogenicity in rats. Chem Biol Interact 2009;181:145"‘53.

Berkson J, Magath TB, Hurn M. The error of estimation of blood cell count as made with the haemocytometer. Ann J Physiol 1940;128:309"‘23.

Haden RL. A new Sahli's type haemoglobinometer. J Lab Clin Med 1939;25:325"‘7.

Wintrob MM. The size and hemoglobin content of the erytrocytes: Methods of determination and clinical application. J Lab Clin Med 1932;17:899"‘912.

Naessens M, Cerdobbel A, Soetaert W, Vandamme EJ. Dextran dextrinase and dextran of Gluconobacter oxydans. J Ind Microbiol Biotechnol 2005;32:323"‘34.

Duncan JR, Prasse KW, Mahaffey EA. Veterinary Laboratory Medicine (Clinical Pathology). Ames: Iowa State University Press; 1994. p. 94"‘6.

Li J, Xia Y, Kuter DJ. Interaction of thrombopoietin with the platelet complements receptor in plasma: Binding, internalization, stability and pharmacokinetics. Br J Haematol 1999;106:345"‘56.

McLellan SA, McLellan DB, Walsh TS. Anaemia and red blood cell transfusion in the critically ill patient. Blood Rev 2003;17:195"‘208.

Dacie FV, Lewis SM. Practical Haematology. 7th ed. Edinburg: Churchill Livingstone; 1995. p. 24.

Ihedioha JI, Chineme CN. Fundamentals of systemic veterinary pathology. Vol. 1. Nsukka: Great AP Express Publishers Ltd; 2004. p. 109"‘28.

Meyer DJ, Coles EH, Rich LJ. Veterinary laboratory medical interpretation and diagnosis. Philadelphia: W.B. Saunders Company; 1992. p. 13"‘40.

Moore KL, Dalley AF. Clinical Oriented Anatomy. 4th ed. Philadelphia: Lippincot Williams and Williams. A Woller Klumner Corporation. 1999. p. 263"‘71.

Price VE, Greenfield RE. Anemia in cancer. In: Advances in Cancer Research. Vol. 5. New York: Academic Press; 1958. p. 199.

Williams N, Levine RF. The origin, development and regulation of megakaryocytes. Br J Haematol 1982;52:173"‘80.

Adedapo AA, Abatan MO, Olorunsogo OO. Toxic effects of someplants in the genus Euphorbia on haematological and biochemical parameters in rats. Veterinarski Arhi 2004;74:53"‘62.

Albers GW. Antithrombotic agents in cerebral ischemia. Am J Cardiol 1995;75:34"‘8B.

George JN. Platelets. Lancet 2005;355:1531"‘9.

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