Isolation, Characterization and Elucidation of Invigorative Potential of Flavonoid From Stem-Bark of Prosopis cineraria on LPS-induced Oxidative Stress and Inflammatory Cascade in Swiss Albino Male Mice

Jump To References Section

Authors

  • Veena Sharma
     Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali – 304022, Rajasthan ,IN
  • Preeti Sharma
     Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali – 304022, Rajasthan ,IN

DOI:

https://doi.org/10.18311/ti/2020/v27i3&4/25615

Keywords:

Anti-inflammatory, Cytokines, Flavonoids, Oxidative Stress, Prosopis Cineraria, ROS

Abstract

The present research aimed to elucidate the structure and characterize the isolated compound from stem-bark of Prosopis cineraria and unravel its potential against LPS-induced toxicity in mouse model. The spectral techniques were done for characterization and structure elucidation of the isolated compound (HPLC, NMR, FT-IR, LC-MS. The experimental mice were intoxicated (intra-peritoneal) with LPS (2 mg/kg body weight) and further treated with isolated compound from Prosopis cineraria (15 mg/kg body weight). Dexamethasone was used as a standard (10 mg/kg body weight). The oxidative stress parameters (LPO, CAT, SOD, GSH, GST and GPx) and biochemical activities (AST, ALT, ACP and ALP) were studied. The levels of pro-inflammatory cytokines (TNF-α; Prostaglandins E2; IL-6; NF-κBp65; IFN-γ and IL-10) were determined in liver homogenate. Nitric Oxide (NO) produced due to LPS-intoxication was determined by using Griess reagent. The results of the spectral analysis were used to elucidate the structure of the isolated flavonoid. The isolated flavonoid suppressed the over-expression and altered levels of oxidative parameters and cytokines due to LPS intoxication and restored the levels of TNF-α, NF-κB, NO, IL-6, IFN- , Prostaglandin E2 and IL-10. The research investigation unfolded the alleviating potential of the isolated compound against LPS-induced adverse effects by modulating the expression of cytokines and combating oxidative stress.

Downloads

Download data is not yet available.

Downloads

Published

2022-08-12

How to Cite

Sharma, V., & Sharma, P. (2022). Isolation, Characterization and Elucidation of Invigorative Potential of Flavonoid From Stem-Bark of <i>Prosopis cineraria</i> on LPS-induced Oxidative Stress and Inflammatory Cascade in Swiss Albino Male Mice. Toxicology International, 27(3&amp;4), 134–148. https://doi.org/10.18311/ti/2020/v27i3&4/25615

Issue

Volume 27, Issue 3&4, July-December 2020

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2020-07-07
Accepted 2020-11-17
Published 2022-08-12

 

References

Singer M, Deutschman CS, Seymour CW. The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA. 2016; 315(8):801–10. PMid:26903338PMCid:PMC4968574. https://doi.org/10.1001/jama.2016.0287

Callery MP, Kamei T, Flye MW. Kupffer cell blockade increases mortality during intra-abdominal sepsis despite improving systemic immunity. Arch Surg. 1990;125(1):36–41.PMid:2294881. https://doi.org/10.1001/archsurg.1990.01410130038005

Nostro A, Germano MP, D'angelo V, Marino A, Cannatelli MA. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett.o Appl. Microbiol. 2000; 30:379. PMid: 10792667.

https://doi.org/10.1046/j.1472-765x.2000.00731.x

Nandkarni KM. Indian Materia Medica. Vol. 1. Popular Prakashan: Mumbai; 2000.

Mohammad IS, Muhammad H, Shoaib Khan NA, Rasool F. Biological potential and phytochemical evaluation of Prosopis cineraria. World Appl Sci J. 2013; 27:1489–94.

Sumathi S, Dharani B, Sivaprabha J. Cell death induced by methanolic extract of Prosopis cineraria leaves in MCF-7 breast cancer cell line. Int J Pharmacol Sci Invent. 2013; 2:21–6.

Nwanjo HU, Ojiako OA. Effect of vitamins E and C on exercise induced oxidative stress. Global J Pure Appl Sci. 2005; 12(2):199–202. https://doi.org/10.4314/gjpas.v12i2.16591

Marklund S, Marklund G. Involvement of superoxide anion radical in the auto-oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47(3):469–74. PMid: 4215654. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x

Aebi H. Catalase in vitro. Methods Enzymol. 1984; 105:121–6. https://doi.org/10.1016/S0076-6879(84) 05016-3

Reitman S, Frankel AS. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. American J Clin Pathol. 1957; 28:53–6. PMid: 13458125. https://doi.org/10.1093/ ajcp/28.1.56

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82:70–7. https://doi.org/10.1016/00039861(59)90090-6

Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974; 249(22):7130–9.

Rotruck JT, Pope AL, Ganther HE. Selenium: Biochemical role as a component of Glutathione Peroxidases. Science. 1973; 179(4073):588–90. PMid: 4686466. https://doi.org/10.1126/science.179.4073.588

Lowry OH, Rosenbrough AL, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem. 1951; 193:265–75.

Sadashivam S, Manickam A. Biochemical methods. Vol 2. New Delhi, India: New Age International (P) Limited; 1996.

Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite,and (15N) nitrate in biological fluids. Anal Biochem. 1982;126:131–8. https://doi.org/10.1016/0003-2697(82) 90118-X

Esterbauer H, Dieber-Rotheneder M, Waeg G, Striegl G, Juergens G. Biochemical structural and functional properties of oxidized low-density lipoprotein. Chem Res Toxicol. 1990; 3(2):77–92. PMid: 2130945. https://doi.org/10.1021/tx00014a001

Kangralkar VA, Patil SD, Bandivadekar RM. Oxidative stress and diabetes: A review. Intl J Pharm Appl. 2010; 1:38–45.

Yasui K, Baba A. Therapeutic potential of Superoxide Dismutase (SOD) for resolution of inflammation. Inflamm Res. 2006; 55:359–63. PMid: 17122956. https://doi.org/10.1007/s00011-006-5195-y

Bowler RP, Nicks M, Tran K, Tanner G, Chang LY, Young SK. Extracellular superoxide dismutase attenuates lipopolysaccharide induced neutrophilic inflammation. Am J Respir Cell Mol Biol. 2004; 31:432–9. PMid: 15256385. https://doi.org/10.1165/rcmb.2004-0057OC

Joseph A, Li Y, Koo HC, Davis JM, Pollack S, Kazzaz JA. Superoxide dismutase attenuates hyperoxia-induced interleukin-8 induction via AP-1. Free Radic Biol Med. 2008; 45:1143–9. PMid: 18692129. https://doi.org/10.1016/j.freeradbiomed.2008.07.006

Porfire AS, LeucuÅ£a SE, Kiss B, Loghin F, Pí¢rvu AE. Investigation into the role of Cu/Zn-SOD delivery system on its antioxidant and anti-inflammatory activity in rat model of peritonitis. Pharmacol Rep. 2014; 66:670–6. PMid: 24948070. https://doi.org/10.1016/j.pharep.2014.03.011

Perez-Rivero G, Ruiz-Torres MP, Diez-Marques ML, Canela A, Lopez-Novoa JM, Rodriguez-Puyol M. Telomerase deficiency promotes oxidative stress by reducing catalase activity. Free Radic Biol Med. 2008; 45:1243–51. PMid: 18718525. https://doi.org/10.1016/j.freeradbiomed.2008.07.017

Ito K, Nakazato T, Yamato K, Miyakawa Y, Yamada T, Hozumi N. Induction of apoptosis in leukemic cells by homovanillic acid derivative, capsaicin, through oxidative stress: Implication of phosphorylation of p53 at Ser-15 residue by reactive oxygen species. Cancer Res. 2004; 64:1071–8. PMid: 14871840. https://doi.org/10.1158/0008-5472.CAN-03-1670

Yu L, Wan F, Dutta S, Welsh S, Liu Z, Freundt E. Autophagic programmed cell death by selective catalase degradation. Proc Natl Acad Sci USA, 2006; 103:4952–7. PMid: 16547133 PMCid: PMC1458776. https://doi.org/10.1073/pnas.0511288103

Abdalla MY. Glutathione as potential target for cancer therapy; more or less is good? (mini-review). Jordan J Biol Sci. 2011; 4(3):119–24.

Nada SA, El-Shamarka ME-S, Omara EA, Abdel-Salam OM. Grape seed extract and Vitamin C combination blocked LPS-induced multiple organ toxicity in mice. ROS. 2019; 7(21):161–75. https://doi.org/10.20455/ ros.2019.827

Jahan MS, Vani G, Shyamaladevi CS. Anti-carcinogenic effect of solanum trilobatum in diethylnitrosamine induced and Phenobarbital promoted hepatocarcinogenesis in rats. Asian J Biochem. 2011; 6(1):74–81. https://doi.org/10.3923/ajb.2011.74.81

Han YJ, Kwon YG, Chung HT. Antioxidant enzymes suppress Nitric Oxide production through the inhibition of NF-kappa B activation: role of H2O2 and Nitric Oxide in inducible Nitric Oxide synthase expression in macrophages. Nitric Oxide. 2001; 5(5):504–13. PMid: 11587565.

RH Shih, CY Wang, CM Yang. NF-kappaB signaling pathways in neurological inflammation: A mini review. Front. Mol Neurosci, 2015; 8:77. PMid: 26733801 PMCid:PMC4683208. https://doi.org/10.3389/fnmol.2015.00077

Choi YY, Kim MH, Hong J, Kim SH, Yang WM. Dried ginger (Zingiber officinalis) inhibits inflammation in alipopolysaccharide- inducedmousemodel.Evid-based Complementary Altern Med. 2013; 914563. PMid: 23935687 PMCid: PMC371222 https://doi.org/10.1155/2013/914563

Park HS, Jung HY, Park EY, Kim J, Lee WJ, Bae YS. Cutting edge: Direct interaction of TLR4 with NAD(P) H oxidase 4 isozyme is essential for lipopolysaccharideinduced production of reactive oxygen species and activation of NF-kappa B. J Immunol. 2004; 173(6):3589–93.PMid: 15356101. https://doi.org/10.4049/jimmunol.173.6.3589

Erridge C, Bennett-Guerrero E, Poxton IR. Structure and function of lipopolysaccharides. Microbes Infect. 2002; 4(8):837–51. https://doi.org/10.1016/ S1286-4579(02)01604-0

Kirtikar KR, Basu BD. Indian medicinal plants Vol. 2. Dehradun India: International Book Distributors; 1984.

Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J Agric Food Chem. 1998; 46:41137. https://doi.org/10.1021/jf9801973

Khatri A, Rathore A, Patil UK. Assessment of antiinflammatory activity of bark of Prosopis cineraria (L.) Druce. Int J Pharm Res. 2012; 4(2):27–9.

Sachdeva S, Kaushik V, Saini V. A review on phytochemical and pharmacological potential of Prosopis cineraria. Int J Ethnobiol Ethnomed. 2014; 1(1):1–4.

Rotelli AE, Guardia T, Juarez AO, de la Rocha NE, Pelzer LE. Comparative study of flavonoids in experimental models of inflammation. Pharmacol Res. 2003; 48:601–6. https://doi.org/10.1016/S1043-6618(03)00225-1

Galvez J, de la Cruz JP, Zarzuelo A, Sanchez de Medina FJ, Jimenez J Sanchez, de la Cuesta F. Oral administration of quercitrin modifies intestinal oxidative status in rats. Gen Pharmacol. 1994; 25:1237– 43. https://doi.org/10.1016/0306-3623(94)90143-0

Comalada M, Camuesco D, Sierra S, Ballester I, Xaus J, Galvez J, Zarzuelo A. In vivo quercetin antiinflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF-kappaB pathway. Eur J Immunol. 2005; 35:584–92. PMid: 15668926. https://doi.org/10.1002/eji.200425778

Sawatzky D, Willoughby D, Colville-Nash P, Rossi A. The involvement of the apoptosis-modulating proteins Erk 1/2, Bcl-xL and Bax in the resolution of acute inflammation in vivo. Am J Pathol. 2006; 168:33–41. PMid: 16400007 PMCid: PMC1592663. https://doi.org/10.2353/ajpath.2006.050058

Most read articles by the same author(s)